×

Dual color camera microscope and methodology for cell staining and analysis

  • US 4,998,284 A
  • Filed: 02/24/1989
  • Issued: 03/05/1991
  • Est. Priority Date: 11/17/1987
  • Status: Expired due to Term
First Claim
Patent Images

1. A method of analyzing cells, having at least a first cellular component and a second cellular component, said method comprising:

  • chemically-optically enhancing the cells with a first and a second spectral stain material,each of said first and second spectral stain materials combining with one of said first and second cellular components to form a first cell combination and a second cell combination,said first cell combination having a maximum optical transmittance at a first predetermined wavelength and a lower optical transmittance at a predetermined second wavelength,said second cell combination having a substantial light absorption at said first predetermined wavelength,transmitting an image of at least the first and second cell combinations, and communicating said image to a beam splitter;

    splitting said image into a first image and a second image;

    filtering the first image with a first filter to provide a first substantially monochromatic image at said first wavelength;

    filtering the second monochromatic image with the second filter to provide a second substantially monochromatic image at said second wavelength;

    sensing the filtered first monochromatic image with a first sensing means to provide a first electrical output representative of the first monochromatic image;

    sensing the filtered second monochromatic image with a second sensing means to provide a second electrical output representative of the second monochromatic image; and

    ,providing an analysis of characteristics of the cells at a characteristic analyzer based on the electrical outputs from the first and second sensing operations.

View all claims
  • 7 Assignments
Timeline View
Assignment View
    ×
    ×