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Method for x and y spermatozoa separation

  • US 4,999,283 A
  • Filed: 08/18/1989
  • Issued: 03/12/1991
  • Est. Priority Date: 01/10/1986
  • Status: Expired due to Term
First Claim
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1. The method for separating male and female determining spermatozoa for the purpose of controlling the sex of offspring prior to conception, comprising the steps of:

  • exposing the spermatozoa in a protein free diluent to an excess concentration of a first antibody that binds male determining spermatozoa;

    suspending said exposed spermatozoa together with a conjugate of a second antibody that binds exclusively to said first antibody and an immunoabsorbent substrate in a protein free diluent to form a conjugate/spermatozoa preparation; and

    recovering the female determining spermatozoa from the conjugate/spermatozoa preparation while said male determining spermatozoa are bound to said immunoabsorbent substrate.

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