Method for x and y spermatozoa separation
First Claim
1. The method for separating male and female determining spermatozoa for the purpose of controlling the sex of offspring prior to conception, comprising the steps of:
- exposing the spermatozoa in a protein free diluent to an excess concentration of a first antibody that binds male determining spermatozoa;
suspending said exposed spermatozoa together with a conjugate of a second antibody that binds exclusively to said first antibody and an immunoabsorbent substrate in a protein free diluent to form a conjugate/spermatozoa preparation; and
recovering the female determining spermatozoa from the conjugate/spermatozoa preparation while said male determining spermatozoa are bound to said immunoabsorbent substrate.
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Abstract
A method for separating male and female determining spermatozoa includes the initial step of exposing freshly ejaculated spermatozoa in a substantially protein free diluent to an excess concentration of a monoclonal antibody directed against H-Y antigen that binds substantially exclusively with male determining spermatozoa. The method continues with the suspending of the exposed spermatozoa together with a conjugate of (1) an immunoglobulin G antibody that binds substantially exclusively to the monoclonal antibody and (2) an immunoabsorbant substrate in a substantially protein free diluent. This forms a conjugate/spermatozoa preparation. The method concludes with the recovering of the separated male and female determining spermatozoa.
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Citations
17 Claims
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1. The method for separating male and female determining spermatozoa for the purpose of controlling the sex of offspring prior to conception, comprising the steps of:
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exposing the spermatozoa in a protein free diluent to an excess concentration of a first antibody that binds male determining spermatozoa; suspending said exposed spermatozoa together with a conjugate of a second antibody that binds exclusively to said first antibody and an immunoabsorbent substrate in a protein free diluent to form a conjugate/spermatozoa preparation; and recovering the female determining spermatozoa from the conjugate/spermatozoa preparation while said male determining spermatozoa are bound to said immunoabsorbent substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification