Production of human pluripotent granulocyte colony-stimulating factor

• US 4,999,291 A
• Filed: 03/06/1989
• Issued: 03/12/1991
• Est. Priority Date: 08/23/1985
• Status: Expired due to Term

• Current Assignee: Amgen Inc.
• Original Assignee: Amgen Inc.
• Inventors: Souza, Lawrence M.
• Primary Examiner(s): Schwartz, Richard A.
• Assistant Examiner(s): Ellis, Joan
• Application Number:
  US07/319,919
• Time in Patent Office: 736 Days
• Field of Search: 435/69.1, 435/69.5, 435/70.3, 435/71.2, 435/91, 435/172.3, 435/320, 536/27, 530/350, 935/13, 935/18, 935/27, 935/32, 935/41, 935/56, 935/58, 935/60, 935/70, 935/73, 935/81
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/535 Granulocyte CSF; Granulocyte-macrophage CSF
• CPC Class Codes: C07K 16/243 Colony Stimulating Factors

Abstract

Disclosed are novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties of a mammalian (e.g., human) pluripotent granulocyte colony-stimulating factor ("hpG-CSF") which are characterized in preferred forms by being the product of procaryotic or eucaryotic host expression of an exogenous DNA sequence. Sequences coding for part or all of the sequence of amino acid residues of hpG-CSF or for analogs thereof may be incorporated into autonomously replicating plasmid or viral vectors employed to transform or transfect suitable procaryotic or eucaryotic host cells such as bacteria, yeast or vertebrate cells in culture. Products of expression of the DNA sequences display, e.g., the physical and immunological properties and in vitro biological activities of isolates of hpG-CSF derived from natural sources. Disclosed also are chemically synthesized polypeptides sharing the biochemical and immunological properties of hpG-CSF.

Claims

1. A purified and isolated DNA sequence consisting essentially of a DNA sequence encoding human pluripotent granulocyte colony-stimulating factor.

2. A purified and isolated DNA sequence according to claim 1 wherein said DNA sequence is a cDNA sequence.

3. A plasmid or viral DNA vector comprising a DNA sequence according to claim 1.

4. A procaryotic or eucaryotic host cell stably transformed or transfected with a DNA sequence according to claim 1 in a manner allowing expression of pluripotent colony-stimulating factor.

5. A process for the production of a polypeptide product, said process comprising:

growing under suitable nutrient conditions, procaryotic or eucaryotic host cells transformed or transfected with a DNA sequence encoding human pluripotent granulocyte colony-stimulating factor in a manner allowing expression of said polypeptide produce, and

isolating desired polypeptide produce of the expression of said DNA sequence.