Production of human pluripotent granulocyte colony-stimulating factor
First Claim
1. A purified and isolated DNA sequence consisting essentially of a DNA sequence encoding human pluripotent granulocyte colony-stimulating factor.
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Abstract
Disclosed are novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties of a mammalian (e.g., human) pluripotent granulocyte colony-stimulating factor ("hpG-CSF") which are characterized in preferred forms by being the product of procaryotic or eucaryotic host expression of an exogenous DNA sequence. Sequences coding for part or all of the sequence of amino acid residues of hpG-CSF or for analogs thereof may be incorporated into autonomously replicating plasmid or viral vectors employed to transform or transfect suitable procaryotic or eucaryotic host cells such as bacteria, yeast or vertebrate cells in culture. Products of expression of the DNA sequences display, e.g., the physical and immunological properties and in vitro biological activities of isolates of hpG-CSF derived from natural sources. Disclosed also are chemically synthesized polypeptides sharing the biochemical and immunological properties of hpG-CSF.
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Citations
5 Claims
- 1. A purified and isolated DNA sequence consisting essentially of a DNA sequence encoding human pluripotent granulocyte colony-stimulating factor.
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5. A process for the production of a polypeptide product, said process comprising:
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growing under suitable nutrient conditions, procaryotic or eucaryotic host cells transformed or transfected with a DNA sequence encoding human pluripotent granulocyte colony-stimulating factor in a manner allowing expression of said polypeptide produce, and isolating desired polypeptide produce of the expression of said DNA sequence.
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Specification