Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes

• US 5,000,000 A
• Filed: 05/15/1989
• Issued: 03/19/1991
• Est. Priority Date: 08/31/1988
• Status: Expired due to Term

• Current Assignee: University of Florida Research Foundation, Incorporated (State University System of Florida)
• Original Assignee: University of Florida (State University System of Florida)
• Inventors: Conway, Tyrrell, Alterthum, Flavio, Ingram, Lonnie O.
• Primary Examiner(s): Teskin, Robin L.
• Application Number:
  US07/352,062
• Time in Patent Office: 673 Days
• Field of Search: 435/161, 435/170, 435/172.3, 435/320, 435/253, 435/252.33, 435/252.3, 536/27, 935/27, 935/14, 935/40, 935/60, 935/41, 935/29, 935/73
• US Class Current: 435/161
• CPC Class Codes: C12N 15/52 Genes encoding for enzymes or proenzymes
• CPC Class Codes: C12P 7/065 with microorganisms other than yeasts
• CPC Class Codes: F25B 2313/02731 using one three-way valve
• CPC Class Codes: F25B 2313/02732 using two three-way valves
• CPC Class Codes: F25B 2313/02741 using one four-way valve
• CPC Class Codes: F25B 2313/02743 using three four-way valves
• CPC Class Codes: G06T 1/00 General purpose image data processing
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A novel operon and plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase activities of Zymomonas mobilis are described. Also disclosed are methods for increasing the growth of microorganisms or eukaryotic cells and methods for reducing the accumulation of undesirable metabolic products in the growth medium of microorganisms or cells.

Claims

1. An Escherichia coli, which has been transformed with Zymomonas mobilis genes coding for alcohol dehydrogenase and pyruvate decarboxylase wherein said genes are expressed at sufficient levels to confer upon said Escherichia coli transformant the ability to produce ethanol as a fermentation product.

2. The Escherichia coli, according to claim 1, wherein the Escherichia coli, prior to transformation, is selected from the group consisting of ATCC 8677, ATCC 8739, ATCC 9637, ATCC 11303, ATCC 11775, ATCC 14948, ATCC 15224, and ATCC 23227.

3. The Escherichia coli, according to claim 1, wherein said Escherichia coli has been transformed with a plasmid selected from the group consisting of pLOI308-10, pLOI297, and pLOI308-11.

4. The Escherichia coli, according to claim 3, wherein said Escherichia coli has been transformed with pLOI297.

5. A method for the production of ethanol, said method comprising transforming an Escherichia coli with Zymomonas mobilis genes coding for pyruvate decarboxylase and alcohol dehydrogenase wherein said genes are expressed by the transformed Escherichia coli at sufficient levels to result in the production of ethanol as a fermentation product when said Escherichia coli is grown in an appropriate medium.

6. The method, according to claim 5, wherein said Escherichia coli is transformed with a plasmid selected from the group consisting of pLOI308-10, pLOI297, and pLOI308-11.

7. The method, according to claim 6, wherein said Escherichia coli has been transformed with pLOI297.