Method for monitoring glucose
First Claim
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1. A method for detecting an elevated glucose level in a body fluid comprising:
- (a) contacting a body fluid containing glucose with a first solid support having immobilized thereon a first conjugate comprising active glucose oxidase and a fluorescent dye, fluorescence emission from said dye being sensitive to oxygen quenching, and with a second solid support having immobilized thereon a second conjugate comprising inactive glucose oxidase and the same aforementioned fluorescent dye whereby said glucose is oxidized by oxygen in said fluid at said first conjugate but not at said second conjugate;
(b) applying excitation light to said first and second conjugates;
(c) detecting fluorescence emission from said first and second conjugates;
(d) comparing the intensities of emission from said first and second conjugates; and
(e) determining that an elevated glucose level exists in said fluid if said intensity of emission from said first conjugate is greater than said intensity of emission from said second conjugate.
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Abstract
A method for monitoring the glucose level in a body fluid uses an apparatus which includes a conjugate of glucose oxidase and a fluorescent dye coated onto an optical fiber in contact with the body fluid, a source of excitation light and a fluorescence emission detector. Glucose is oxidized by oxygen in the body fluid causing a decrease in oxygen concentration at the enzyme. The fluorescent dye is sensitive to oxygen quenching so that, when the oxygen concentration decreases, fluorescence emission increases in direct proportion to the glucose concentration in the fluid.
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Citations
9 Claims
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1. A method for detecting an elevated glucose level in a body fluid comprising:
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(a) contacting a body fluid containing glucose with a first solid support having immobilized thereon a first conjugate comprising active glucose oxidase and a fluorescent dye, fluorescence emission from said dye being sensitive to oxygen quenching, and with a second solid support having immobilized thereon a second conjugate comprising inactive glucose oxidase and the same aforementioned fluorescent dye whereby said glucose is oxidized by oxygen in said fluid at said first conjugate but not at said second conjugate; (b) applying excitation light to said first and second conjugates; (c) detecting fluorescence emission from said first and second conjugates; (d) comparing the intensities of emission from said first and second conjugates; and (e) determining that an elevated glucose level exists in said fluid if said intensity of emission from said first conjugate is greater than said intensity of emission from said second conjugate. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for detecting an elevated glucose level in a body fluid comprising:
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(a) contacting a body fluid containing glucose with first, second, third and fourth optical fibers, said first and second fibers having immobilized thereon a first covalent conjugate of glucose oxidase and a fluorescent dye, fluorescence emission from said dye being subject to oxygen quenching, said first conjugate being coated with a membrane selectively permeable to molecules the size of glucose and smaller, said third and fourth optical fibers having immobilized thereon a second covalent conjugate of glucose oxidase and the same aforementioned fluorescent dye, said second conjugate being coated with a membrane selectively permeable to oxygen but impermeable to glucose, whereby said glucose is oxidized by oxygen in said fluid at said first conjugate ,but not at said second conjugate; (b) applying excitation light to said first conjugate through said first fiber and to said second conjugate through said third fiber; (c) detecting fluorescence emission from said first conjugate through said second fiber and from said second conjugate through said fourth fiber; (d) comparing the intensity of emission from said first conjugate with the intensity of emission from said second conjugate; and (e) determining that an elevated glucose level exists in said fluid if said intensity of emission from said first conjugate is greater than said intensity of emission from said second conjugate.
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9. A method for quantitating an elevated concentration of glucose in a body fluid comprising:
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(a) contacting a body fluid containing glucose with a covalent conjugate comprising immobilized active glucose oxidase and a fluorescent dye, fluorescence emission from said dye being sensitive to oxygen quenching, whereby glucose is oxidized by oxygen in said fluid at said conjugate; (b) applying excitation light to said conjugate; (c) measuring fluorescence emission from said conjugate; (d) quantitating an elevated concentration of glucose in a body fluid by comparing the magnitude of said emission with the magnitude of fluorescence emission measured when a fluid containing a known quantity of glucose is subjected to steps (a) to (c).
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Specification