Detectable molecules, method preparation and use
First Claim
1. A compound of the formula
whereA3 is A2 or a polymer, both A1 and the polymer having at least one modifiable reactive group consisting of amino, hydroxy, cis OH, halide, aryl, imidazoyl, carbonyl, carboxyl, thiol or a residue comprising an activated carbon;
A2 is a specific ligand having a molecular weight of less than about 2,000;
--X-- is selected from the group consisting of ##STR51## a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH;
E is OH, NH or an acyclic divalent sulfur atom--Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where --R2 -- is a C1 -C10 branched or unbranched alkyl; and
Za is chlorine, bromine or iodine.which compound has at least one couplable reactive site thereon.
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Accused Products
Abstract
A detectable molecule of the formula
A.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.m
where A3 is A2 or a polymer, where A3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where R2 is a C1 -C10 branched or unbranched alkyl; Za is chlorine, bromine or iodine; E is O, NH or an acylic divalent sulfur atom; Detb is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR2## or the 4-hydroxy or acyloxy derivative thereof, where R3 is C1 -C4 alkyl or CH2 COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C1 -C10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A3. The detectable molecules are useful in in vitro or in vivo assays or therapy.
101 Citations
24 Claims
- 1. A compound of the formula
- space="preserve" listing-type="equation">A.sup.3 --(--X--R.sup.1 --E--).sub.m
where A3 is A2 or a polymer, both A1 and the polymer having at least one modifiable reactive group consisting of amino, hydroxy, cis OH, halide, aryl, imidazoyl, carbonyl, carboxyl, thiol or a residue comprising an activated carbon; A2 is a specific ligand having a molecular weight of less than about 2,000; --X-- is selected from the group consisting of ##STR51## a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; E is OH, NH or an acyclic divalent sulfur atom --Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where --R2 -- is a C1 -C10 branched or unbranched alkyl; and Za is chlorine, bromine or iodine. which compound has at least one couplable reactive site thereon. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
Specification
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- Resources
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Current AssigneeEnzo Diagnostics, Inc.
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Original AssigneeEnzo Biochem Incorporated
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InventorsStavrianopoulos, Jannis G.
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Primary Examiner(s)NUCKER, CHRISTINE M
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Application NumberUS07/513,723Time in Patent Office336 DaysField of Search530/350, 530/402, 530/387, 530/389, 530/390, 530/391, 435/188, 435/7, 435/6, 536/27-29, 536/1.1, 536/55.1, 536/56, 536/102, 436/548US Class Current435/188CPC Class CodesA61K 51/0497 conjugates with a carrier b...A61K 51/1093 conjugates with carriers be...G01N 33/533 with fluorescent labelG01N 33/553 Metal or metal coated
