Reaction well shape for a microwell tray

• US 5,002,889 A
• Filed: 10/21/1988
• Issued: 03/26/1991
• Est. Priority Date: 10/21/1988
• Status: Expired due to Fees

• Current Assignee: Genetic Systems Corp. (Bio-Rad Laboratories Incorporated)
• Original Assignee: Genetic Systems Corp. (Bio-Rad Laboratories Incorporated)
• Inventors: Klein, Gerald L.
• Primary Examiner(s): Dority, Carroll B.
• Application Number:
  US07/260,836
• Time in Patent Office: 886 Days
• Field of Search: 435/296, 435/287, 435/284, 435/293, 435/301, 215/1 R, D 7/6, 422/102
• US Class Current: 435/305.2
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates

Abstract

A microwell for enzyme-linked immunosorbent assays has a concavely curved, circumferential sidewall, a flat optical bottom and a top lip, including smooth transitions between the top lip, concave sidewall and flat bottom. This shape minimizes the tendency of fluid to cling to the well for washing efficiency and maximizes a vertical optical path length of fluid in the well for improved optical determination.

Claims

1. An apparatus comprising a molded device having a plurality of reaction wells adapted for photometric determination of a fluid characteristic, each reaction well having a volume capacity of less than one milliliter and comprising;

a convexly curved, circumferential top lip centered about a vertical well axis and defining an opening for the well;

a concavely curved, circumferential sidewall centered about the well axis and contiguous with the top lip and having a radiums of curvature of approximately 0.38 inch as measured about the vertical well axis approximately 0.223 inches above a well bottom;

a concavely curved, circumferential transition wall centered about the well axis and contiguous with the circumferential sidewall; and

a circular, planar optical window centered about the well axis and contiguous with the transition wall so as to define the bottom for the well, wherein the circumferential top lip and circumferential transition wall have radii of curvature substantially less than the radius of curvature of the circumferential sidewall.

2. The reaction well of claim 1 wherein the circumferential sidewall has a parabolic shape.

3. The reaction well of claim 1 wherein the circumferential sidewall is a surface of revolution.

4. The reaction well of claim 1 wherein the circumferential top lip and circumferential transition wall have a radius of curvature of approximately 0.012 inch.

5. The reaction well of claim 4 wherein the circumferential top lip has a diameter of approximately 0.22 inch.

6. The reaction well of claim 5 wherein the optical window has a diameter of approximately 0.059 inch.

7. The reaction well of claim 6 wherein the well has a height, defined by a distance between the circumferential top lip and the optical window, of approximately 0.180 inch measured along the well axis.

8. The reaction well of claim 1 wherein the circumferential top lip and circumferential sidewall have tangents, at respective contiguous portions thereof, which are parallel.

9. The reaction well of claim 1 wherein the circumferential sidewall and circumferential transition wall have tangents, at respective contiguous portions thereof, which are parallel.

10. The well shape of claim 1 wherein the circumferential sidewalls, and the circumferential top lip and transition wall, have tangents, at respective contiguous portions thereof, which are parallel.

14. The apparatus of claim 1, wherein the reaction wells are combined in a row to form a strip.

11. An apparatus comprising a molded device having a plurality of reaction wells for photometric determination of a fluid characteristic, each well having a volume capacity of less than one milliliter and the shape of the well comprising;

a circumferential top lip defining an open top for the well;

a peripheral, substantially concavely curved sidewall contiguous with the top lip and having a smooth transition therebetween and having a radius of curvature of approximately 0.38 inch as measured above a well bottom; and

axis approximately 0.223 inches above a well bottom; and

a substantially flat optical window connected to the curved sidewall and having a smooth transition there between defining the bottom for the well.

12. The well shape of claim 11 wherein the curved sidewall has a parabolic curve.

13. The well shape of claim 11 wherein the curved a sidewall has a spherical curve.

15. The apparatus of claim 11, wherein the reaction wells are combined in a row to form a strip.

16. The apparatus of claim 11, wherein the wells are formed in a place.