Reaction well shape for a microwell tray
First Claim
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1. An apparatus comprising a molded device having a plurality of reaction wells adapted for photometric determination of a fluid characteristic, each reaction well having a volume capacity of less than one milliliter and comprising;
- a convexly curved, circumferential top lip centered about a vertical well axis and defining an opening for the well;
a concavely curved, circumferential sidewall centered about the well axis and contiguous with the top lip and having a radiums of curvature of approximately 0.38 inch as measured about the vertical well axis approximately 0.223 inches above a well bottom;
a concavely curved, circumferential transition wall centered about the well axis and contiguous with the circumferential sidewall; and
a circular, planar optical window centered about the well axis and contiguous with the transition wall so as to define the bottom for the well, wherein the circumferential top lip and circumferential transition wall have radii of curvature substantially less than the radius of curvature of the circumferential sidewall.
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Abstract
A microwell for enzyme-linked immunosorbent assays has a concavely curved, circumferential sidewall, a flat optical bottom and a top lip, including smooth transitions between the top lip, concave sidewall and flat bottom. This shape minimizes the tendency of fluid to cling to the well for washing efficiency and maximizes a vertical optical path length of fluid in the well for improved optical determination.
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Citations
16 Claims
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1. An apparatus comprising a molded device having a plurality of reaction wells adapted for photometric determination of a fluid characteristic, each reaction well having a volume capacity of less than one milliliter and comprising;
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a convexly curved, circumferential top lip centered about a vertical well axis and defining an opening for the well; a concavely curved, circumferential sidewall centered about the well axis and contiguous with the top lip and having a radiums of curvature of approximately 0.38 inch as measured about the vertical well axis approximately 0.223 inches above a well bottom; a concavely curved, circumferential transition wall centered about the well axis and contiguous with the circumferential sidewall; and a circular, planar optical window centered about the well axis and contiguous with the transition wall so as to define the bottom for the well, wherein the circumferential top lip and circumferential transition wall have radii of curvature substantially less than the radius of curvature of the circumferential sidewall. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 14)
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11. An apparatus comprising a molded device having a plurality of reaction wells for photometric determination of a fluid characteristic, each well having a volume capacity of less than one milliliter and the shape of the well comprising;
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a circumferential top lip defining an open top for the well; a peripheral, substantially concavely curved sidewall contiguous with the top lip and having a smooth transition therebetween and having a radius of curvature of approximately 0.38 inch as measured above a well bottom; and
axis approximately 0.223 inches above a well bottom; anda substantially flat optical window connected to the curved sidewall and having a smooth transition there between defining the bottom for the well. - View Dependent Claims (12, 13, 15, 16)
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Specification