Single color reading method for determining fructosamine
First Claim
1. A method of determining the level of fructosamine in a blood sample or a sample derived from blood, said method comprising the steps of eliminating any interfering substances from the sample, controlling the pH of the sample to a value between 10 and 14, adding a coloring agent to the sample and, after a delay, taking a color measurement and determining the fructosamine level in said sample by comparing a single color measurement with that of a single color measurement on a standard solution, wherein the coloring agent and pH conditions are selected such that a change of color in the coloring agent is caused by glucose in the sample that has reacted or associated with an amine group of protein and has undergone a molecular rearrangement to form fructosamine.
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Abstract
A single color determination method of quantization of the amount of fructosamine in a sample such as serum by removing other interfering reducing agents and developing a color with a coloring agent such as tetrazolium salt.
26 Citations
8 Claims
- 1. A method of determining the level of fructosamine in a blood sample or a sample derived from blood, said method comprising the steps of eliminating any interfering substances from the sample, controlling the pH of the sample to a value between 10 and 14, adding a coloring agent to the sample and, after a delay, taking a color measurement and determining the fructosamine level in said sample by comparing a single color measurement with that of a single color measurement on a standard solution, wherein the coloring agent and pH conditions are selected such that a change of color in the coloring agent is caused by glucose in the sample that has reacted or associated with an amine group of protein and has undergone a molecular rearrangement to form fructosamine.
- 7. A mehod of determining the fructosamine level in a sample, said method comprising the steps of eliminating any interfering substances from the sample and controlling the pH of the sample to a value between 10 and 14, adding a coloring agent to the sample capable of reacting with fructosamine to develop a color and, after a delay, taking a single color measurement and determining the fructosamine level in said sample by comparing single color measurement with a color developed with said coloring agent and standard fructosamine solution, wherein any reaction between the coloring agent and fructosamine in the sample is stopped by an acid.
Specification