Device for analyzing fluorescent light signals

• US 5,003,977 A
• Filed: 03/09/1989
• Issued: 04/02/1991
• Est. Priority Date: 03/31/1988
• Status: Expired due to Fees

• Current Assignee: Kabushiki Kaisha Square Enix
• Original Assignee: Agency for Industrial Science and Technology, Ministry of International Trade & Industry (Government of Malaysia)
• Inventors: Ishida, Kazuyuki, Ozaki, Takeo, Suzuki, Susumu
• Primary Examiner(s): Cohen, Lee S.
• Assistant Examiner(s): Hanley, John C.
• Application Number:
  US07/321,296
• Time in Patent Office: 754 Days
• Field of Search: 128/633, 128/634, 128/664, 128/665, 128/395, 606/15, 606/16, 606/7, 606/10
• US Class Current: 600/317
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: G01J 2003/2873 Storing reference spectrum
• CPC Class Codes: G01J 3/4406 Fluorescence spectrometry
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16

Abstract

A device for analyzing fluorescent light signals consists of memory means for storing as a reference spectrum a spectrum obtained under predetermined conditions, and extraction means for extracting a predetermined component spectrum based on the results of a conversion of the reference spectrum using a specific wavelength of a measured spectrum obtained during actual measurement and a specific wavelength of the reference spectrum. The arrangement of the invention allows the spectrum of a specific component to be extracted accurately from measured spectra.

Claims

1. A device for analyzing fluorescent light signals, comprising:

first light-irradiating means for irradiating a normal position of an organism with first laser light;

first light-detecting means for detecting a first spectrum of fluorescence excited by the irradiation of said first laser light;

memory means for storing, as a reference spectrum, the first spectrum of fluorescence detected by said first light-detecting means;

second light-irradiating means for irradiating with second laser light a position to be diagnosed of the organism injected with a fluorescent substance having a strong affinity for tumors;

second light-detecting means for detecting a second spectrum of fluorescence excited by the irradiation of said second laser light;

correction means for correcting said reference spectrum on the basis of a ratio of an intensity of a specific wavelength in the second spectrum detected by said second light-detecting means to an intensity of the same wavelength as said specific wavelength in said reference spectrum; and

extraction means for subtracting the corrected reference spectrum from the detected second spectrum and extracting the difference.

2. A device for analyzing fluorescent light signals, comprising:

light-irradiating means for irradiating with a laser a normal position of an organism and a position to be diagnosed of said organism injected with a fluorescent substance having a strong affinity for tumors;

light-detecting means for detecting a first spectrum of fluorescence excited by irradiation of said normal position by said light-irradiating means and a second spectrum of fluorescence excited by irradiation of said diagnosed position by said light-irradiating means;

first memory means for storing said first spectrum, said first spectrum being used as a reference spectrum;

second memory means for storing an intensity at a wavelength in said first spectrum and for storing an intensity of said wavelength in said second spectrum;

correction means for correcting said reference spectrum by multiplying said reference spectrum by a ratio of said intensity at said wavelength in said first spectrum to said intensity at said second wavelength in said spectrum; and

extraction means for subtracting said corrected reference spectrum from said second spectrum and for outputting the difference.

3. A device as recited in claim 2, wherein:

said first spectrum at said wavelength has a much greater intensity than said second spectrum at said wavelength.