Dry-type multilayer analytical element
First Claim
1. A dry multilayer analytical element for the determination of an analyte in an aqueous liquid sample by measuring the amount of hydrogen peroxide produced from the interaction of the analyte with an oxidase, comprising, in this order, a light-transmissive water-impermeable support, a chromogen-containing layer which produces an optically detectable change from the interaction of hydrogen peroxide, and a peroxidatively active substance-containing layer, said element further comprising an oxidase which reacts with the analyte to prouduce hydrogen peroxide, and a ferrocyanide, with the proviso that the ferrocyanide is in a layer separate from the chromogen-containing layer and is not interposed between the support and the chromogen-containing layer.
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Abstract
A dry-type multilayer analytical element containing a color reagent composition comprising an oxidase specifically reaction with an analyte or derived therefrom to produce hydrogen peroxide, hydrogen peroxide-decomposing enzyme, an alkali metal salt of ferrocyanide and a chromogen which is separated from the hydrogen peroxide-decomposing enzyme, the chromogen being incorporated in a layer nearer to the transparent support of the analytical element than the layer containing the hydrogen peroxide-decomposing enzyme. The analytical element exhibits excellent coloration, color stability, and accuracy and the results are minimally influenced by the presence of hemoglobin in the sample.
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Citations
18 Claims
- 1. A dry multilayer analytical element for the determination of an analyte in an aqueous liquid sample by measuring the amount of hydrogen peroxide produced from the interaction of the analyte with an oxidase, comprising, in this order, a light-transmissive water-impermeable support, a chromogen-containing layer which produces an optically detectable change from the interaction of hydrogen peroxide, and a peroxidatively active substance-containing layer, said element further comprising an oxidase which reacts with the analyte to prouduce hydrogen peroxide, and a ferrocyanide, with the proviso that the ferrocyanide is in a layer separate from the chromogen-containing layer and is not interposed between the support and the chromogen-containing layer.
Specification