Flow-cell device

• US 5,007,732 A
• Filed: 04/18/1988
• Issued: 04/16/1991
• Est. Priority Date: 04/20/1987
• Status: Expired due to Term

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Kamohara, Hideaki, Ohki, Hiroshi, Miyake, Ryo
• Primary Examiner(s): Rosenberger, Richard A.
• Assistant Examiner(s): Pham, Hoa
• Application Number:
  US07/182,430
• Time in Patent Office: 1,093 Days
• Field of Search: 356/73, 356/72, 356/353, 356/410, 356/337, 356/343, 356/39, 350/362, 250/257
• US Class Current: 356/73
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing

Abstract

A sheath flow type flow-cell device for plow-cytometer which comprises a first inlet for sheath fluid, a flow passage communicated with the first inlet and contracted toward downstream, the flow passage having a substantially rectangular cross section, a straight capillary flow passage connected to the flow passage downstream thereof, the capillary flow passage having a substantially rectangular cross section, a second inlet for sample fluid, a nozzle communicating with the second inlet and opened within the flow passage in the same direction as the flow direction of the straight capillary flow passage, a discharge port provided at a terminal end of the straight capillary flow passage, and flow regulating means for regulating the flow of the sheath fluid in the straight capillary flow passage to be a laminar flow having a gradient of flow velocity.

Claims

1. A sheath flow type flow-cell device for a flow-cytometer comprising:

a first inlet for sheath fluid,

a flow passage communicating with said first inlet and contracted toward downstream, said flow passage having a substantially rectangular cross section,

a straight capillary flow passage connected to said flow passage downstream thereof, said capillary flow passage having a substantially rectangular cross section,

a second inlet for sample fluid,

a nozzle communicating with said second inlet and opened within said flow passage in the same direction as the flow direction of said straight capillary flow passage,

a discharge port provided at a terminal end of said straight capillary flow passage, and

flow regulating means for regulating the flow of said sheath fluid in said straight capillary flow passage to be a laminar flow having a gradient of flow velocity, wherein said straight capillary flow passage has opposing first and second side walls connected by top and bottom walls and wherein said flow regulating means comprises said second said wall having a rougher surface than a surface of said first said wall.

2. A sheath flow type flow-cell device as claimed in claim 1, wherein said flow regulating means further includes a part of said top wall and a part of said bottom wall of said capillary flow passage having rough surface portions adjacent to said second side wall.

3. A sheath flow type flow-cell device as claimed in claim 2, wherein the width of said rough surfaces of said top and bottom walls from said second side wall is 1/3 and under of a distance between said first side wall and said second side wall.

4. A sheath flow type flow-cell device as claimed in claim 1, wherein the surface roughness of said surface of said first side wall is 1/500 and under of a distance between said first side wall and said second side wall and the surface roughness of said surface of said second side wall is 1/20 and over of said distance.

5. A sheath flow type flow-cell device as claimed in claim 1, wherein the surface roughness of said surface of said first side wall exists from 1 S to 10 S and the surface roughness of said surface of said second side wall exists from 100 S to 1000 S.

6. A sheath flow type flow-cell device for a flow-cytometer comprising:

a first inlet for sheath fluid,

a flow passage communicating with said first inlet and contracted toward downstream, said flow passage having a substantially rectangular cross section,

a straight capillary flow passage connected to said flow passage downstream thereof, said capillary flow passage having a substantially rectangular cross section,

a second inlet for sample fluid,

a nozzle communicating with said second inlet and opened within said flow passage in the same direction as the flow direction of said straight capillary flow passage,

a discharge port provided at a terminal end of said straight capillary flow passage, and

flow regulating means for regulating the flow of said sheath fluid in said straight capillary flow passage to be a laminar flow having a gradient of flow velocity, wherein said flow regulating means comprises a plurality of partition walls extended in said flow passage in the flow direction and dividing said flow passage into a plurality of divided flow passages of which flow resistance are made larger from a first side wall of said flow passage toward a second side wall opposite to said first side wall.

7. A sheath flow type flow-cell device for a flow-cytometer comprising:

a first inlet for sheath fluid,

a flow passage communicating with said first inlet and contracted toward downstream, said flow passage having a substantially rectangular cross section,

a straight capillary flow passage connected to said flow passage downstream thereof, said capillary flow passage having opposing first and second side walls joined by top and bottom walls and having a substantially rectangular cross section,

a second inlet for sample fluid which includes cells therein,

a nozzle communicated with said second inlet and opened within said flow passage in the same direction as the flow direction of said straight capillary flow passage,

a discharge port provided at a terminal end of said straight capillary flow passage, and

flow regulating means for regulating the flow of said sheath fluid in said straight capillary flow passage to be a laminar flow having a gradient of flow velocity from said first side wall to said second said wall which is sufficient to flatten said cells in said sample fluid into a form symmetrical with respect to an axis thereof and having a longitudinal axis coinciding with said flow direction of said straight capillary flow passage.

8. A sheath flow type flow-cell device for a flow-cytometer comprising:

a first inlet for sheath fluid,

a flow passage communicating with said first inlet and contracted toward downstream, said flow passage having a substantially rectangular cross section,

a straight capillary flow passage connected to said flow passage downstream thereof, said capillary flow passage having a substantially rectangular cross section,

a second inlet for sample fluid,

a nozzle communicating with said second inlet and opened within said flow passage in the same direction as the flow direction of said straight capillary flow passage,

a discharge port provided at a terminal end of said straight capillary flow passage, and

flow regulating means for regulating the flow of said sheath fluid in said straight capillary flow passage to be a laminar flow having a gradient of flow velocity, wherein said flow regulating means regulates said flow of said sheath fluid in said straight capillary flow passage to be a laminar flow having a gradient of flow velocity increasing across said straight capillary flow passage from one side thereof to another side thereof opposite said one side.

9. A sheath flow type flow-cell device as claimed in claim 8, wherein said flow regulating means comprises a net member stretched across said flow passage upstream from the opening of said nozzle, the mesh of said net being minuter from a first side wall of said flow passage toward second side wall opposite to said first side wall.