Biogenic amine assay using HPLC-ECD

• US 5,011,608 A
• Filed: 11/18/1988
• Issued: 04/30/1991
• Est. Priority Date: 11/18/1988
• Status: Expired due to Fees

• Current Assignee: Dragana Damjanovic
• Original Assignee: Dragana Damjanovic
• Inventors: Damjanovic, Dragana
• Primary Examiner(s): Fisher, Richard V.
• Assistant Examiner(s): McCarthy, Neil M.
• Application Number:
  US07/273,449
• Time in Patent Office: 893 Days
• Field of Search: 210/198.2, 210/635, 210/656, 436/106, 436/111, 436/112, 436/161, 436/816, 436/901, 73/61.1 C, 422/70
• US Class Current: 210/656
• CPC Class Codes: B01D 15/08 Selective adsorption, e.g. chromatography
• CPC Class Codes: G01N 2030/626 calibration, baseline
• CPC Class Codes: G01N 2030/645 electrical conductivity detectors
• CPC Class Codes: G01N 2030/8813 biological materials
• CPC Class Codes: G01N 30/36 in high pressure liquid systems
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
• CPC Class Codes: G01N 33/9406 Neurotransmitters
• CPC Class Codes: Y10S 436/816 Alkaloids, amphetamines, and barbiturates
• CPC Class Codes: Y10S 436/901 Drugs of abuse, e.g. narcotics, amphetamine
• CPC Class Codes: Y10T 436/173845 Amine and quaternary ammonium

Abstract

A method for assaying compounds belonging to the group biogenic amines and including catecholamines, indoleamines, their metabolites and derivatives, and other small molecular weight compounds using a boric acid extraction method followed by high pressure liquid chromatographic separation in conjunction with electrochemical detection. The method utilizes high purity chemical and liquid components, a microparticulate-silica bonded phenyl stationary phase in the chromatography column and special cleaning and maintenance measures for the various components of the assaying apparatus which result in reduced baseline noise and allow the electrochemical cell to be operated at a sensitivity of one nanoamp or less full scale deflection on a continuous basis.

Claims

1. A method for the assaying of low concentrations of an oxidizable or reducible biogenic amine or derivative or metabolite thereof, comprising the steps of:

(a) preparing a sample of at least one said compound, said sample being suitable for quantitative analysis;

(b) injecting said sample into a high pressure liquid chromatography apparatus to thereby form a chromatogram, said apparatus including;

(1) a chromatography column containing a quantity of microparticulate material as a stationary phase;

(2) a mobile phase passing through said stationary phase, said mobile phase comprising the following components;

(a) from about 50 mM to about 250 mM of a buffer having a pH of from about 2 to about 8;

(b) from about 0.1 mM to about 10.0 mM of a chelating agent;

(c) from about 0.1 mM to about 10.0 mM of an alkyl sulfonate, said alkyl sulfonate having from about 4 to about 14 carbon atoms; and

(d) from about 0.1% to about 10% by volume methanol in water solution;

(3) an injector for injecting said sample;

(4) a pump for pumping said mobile phase through said stationary phase; and

(5) a guard column positioned upstream of said chromatography column;

(c) eluting said compounds from said chromatography column;

(d) passing said eluted compounds through an electrochemical detector cell thereby producing a current output from said detector;

(e) measuring and integrating the current output from said electrochemical detector to obtain a quantitative analysis of said compound; and

(f) maintaining a substantially constant base line noise at a sensitivity of said electrochemical detector cell sufficient to measure the quantity of said compound present in resting, human plasma by maintaining all portions of said apparatus contacting said mobile phase and said stationary phase substantially residue-free, wherein the signal to noise ratio of said current output is at least 2 to 1.

2. A method as claimed in claim 1, wherein said compound is natural or synthetic, and is derived from the fluids and tissues of vertebrates, invertebrates or plants.

3. A method as claimed in claim 2 wherein said fluids and tissues comprise blood, cerebrospinal fluid, lymphatic fluid, urine, other extracellular fluids, tissues or tissue extracts.

4. A method as claimed in claim 3, wherein said fluids and tissues are derived from mammals.

5. A method as claimed in claim 4, wherein said mammals are humans.

6. A method as claimed in claim 4, wherein said fluid is plasma.

7. A method as claimed in claim 6, wherein said plasma is human plasma.

8. A method as claimed in claim 7, wherein said human plasma is collected non-traumatically and under aseptic conditions, and wherein said non-traumatic collection procedures include withdrawing a plasma sample via an indwelling catheter inserted into the subject from whom the plasma is being withdrawn at least ten minutes prior to the withdrawal of the sample.

9. A method as claimed in claim 1, wherein said compound is extracted from a source by the steps of binding said compound contained in said source to a carrier thereby forming a carrier complex, extracting the complex from the source, and recovering the compound from the carrier complex.

10. A method as claimed in claim 9, wherein said carrier comprises a boron derivative.

11. A method as claimed in claim 9, wherein said extraction includes forming a polar complex of said compound and said boron derivative in an aqueous medium, combining said complex in an aqueous medium with an organic solution containing a counterion for said complex, thereby forming a substantially non-polar complex miscible in said organic solution, separating said organic solution containing said nonpolar complex from said aqueous medium, and washing said organic solution with an aqueous acid solution to thereby liberate said compound from said non-polar complex and separating said acid solution containing said compound from said organic solution.

12. A method as claimed in claim 11, wherein said boron derivative is diphenylborate ethanolamine, wherein said counterion is tetraoctylammonium bromide, wherein said aqueous medium is ammonium chloride-ammonium hydroxide buffer, wherein said organic solution is a mixture of heptane and octanol, and wherein said acid is acetic acid.

13. A method as claimed in claim 11 wherein said boron derivative, said aqueous medium, said organic solution, said counterion, and said aqueous acid solution are comprised of chemicals and reagents having a level of impurity no greater than about 10 ppm.

14. A method as claimed in claim 9, wherein said extraction method is monitored by the addition of an internal standard to the sample matrix prior to extraction.

15. A method as claimed in claim 14, wherein said internal standard does not interfere with the extraction method, and wherein said internal standard does not adversely affect the integrity of the compounds being extracted, and wherein said internal standard does not interfere with the discrimination of the peaks of interest on the chromatogram.

16. A method as claimed in claim 15, wherein said internal standard comprises a catecholamine-like compound.

17. A method as claimed in claim 16, wherein said compound is deoxyepinephrine.

18. A method as claimed in claim 9, wherein sample preparation equipment used in extracting said compound from said source includes a friction-reducing coating over all surfaces contacting said compound to thereby reduce the level of adherence of said compound to said surfaces.

19. A method as claimed in claim 18, wherein said friction-reducing material comprises a silicone-containing material.

20. A method as claimed in claim 18, wherein said sample preparation equipment is washed with an acid prior to initial utilization of said equipment in said extraction method, followed by rinsing said equipment with water to remove said acid.

21. A method as claimed in claim 20, said sample preparation equipment is utilized in said method to prepare a plurality of samples by the steps of:

(a) washing said equipment with a substantially residue-free aqueous solution of detergent;

(b) rinsing said washed equipment with distilled water;

(c) preparing said sample with said equipment;

(d) removing said sample from said equipment; and

(e) repeating said detergent washing step and said distilled water rinsing step prior to contacting said equipment with another said sample.

22. A method as claimed in claim 1, including injecting at least one sample into said apparatus, thereby decreasing the level of sensitivity of said electrochemical cell, said sensitivity being thereby increased to a level higher from said decreased level by passing a solution through said apparatus at a superficial velocity of from about 2 to about 200 cm/min. for a period of from about 1 to 15 hours, said solution comprising said mobile phase, the amount of methanol in said solution being less than the threshold amount necessary for disruption of the reference electrode in said cell, and containing sufficient quantity of an alkyl sulfonate so as to flush fatty acids from the apparatus.

23. A method as claimed in claim 1, wherein said method includes cleaning said high pressure liquid chromatography apparatus which comprises the steps, in sequence, of displacing said mobile phase with a first quantity of water, displacing said water with a first quantity of a methanol/water mixture having from about 30 to about 70 vol. % methanol, displacing said methanol/water mixture with methanol, displacing said methanol with a second quantity of said methanol/water mixture, displacing said second quantity of said methanol/water mixture with a second quantity of said water and displacing said second quantity of water with a fresh quantity of said mobile phase.

24. A method as claimed in claim 23, wherein the purity of said water is at least Type I Reagent Grade Water with a minimum resistivity of 10 MΩ

, and wherein said methanol has a level of impurity of no greater than about 10 ppm.

25. A method as claimed in claim 1, wherein said method includes cleaning said high pressure liquid chromatography apparatus by the steps of, in sequence, displacing said mobile phase with a first quantity of water, disconnecting said guard-column, said chromatography column and said detector from the injector and the pump, washing said pump and said injector with a quantity of acid, flushing said acid from said pump and said injector with a quantity of water sufficient to result in a substantially neutral effluent, reconnecting said chromatography column, said guard-column and said detector to said pump and said injector, displacing said water with a first quantity of a methanol/water mixture having from about 30 to about 70 vol. % methanol, displacing said methanol/water mixture with methanol, displacing said methanol with a second quantity of said methanol/water mixture, displacing said second quantity of said methanol/water mixture with a second quantity of said water and displacing said second quantity of water with a fresh quantity of said mobile phase.

26. A method as claimed in claim 25, wherein said acid comprises nitric acid, wherein said water comprises Type I Reagent Grade water with a minimum resistivity of 10 MΩ

, and wherein said methanol has a level of impurity no greater than about 10 ppm.

27. A method as claimed in claim 1, wherein said chromatography column comprises a microparticulate bonded silica.

28. A method as claimed in claim 27, wherein said microparticulate bonded silica comprises alkylphenyl groups having from 7 to 12 carbon atoms bonded to silica particles.

29. A method as claimed in claim 28, wherein said silica particles are irregularly-shaped.

30. A method as claimed in claim 29, wherein said irregularly-shaped silica particles are about 10 μ

in size.

31. A method as claimed in claim 28, wherein said alkylphenyl is ethylphenyl.

32. A method as claimed in claim 1, wherein said method further includes filtering said mobile phase, degassing said filtered mobile phase, and purging said degassed mobile phase with an inert gas prior to passing said mobile phase through said stationary phase.

33. A method as claimed in claim 32, wherein said filter comprises a membrane filter having a pore size of at least about 0.22 μ

.

34. A method as claimed in claim 32, wherein said inert gas comprises nitrogen or a noble gas.

35. A method as claimed in claim 1, wherein said buffer, said chelating agent, said alkyl sulfonate, said methanol and said water in said mobile phase each have a level of impurity of no greater than about 10 ppm.

36. A method as claimed in claim 1, wherein said buffer is sodium phosphate having a pH of about 4.8, and wherein said sodium phosphate buffer has a concentration of about 70 mM.

37. A method as claimed in claim 1, wherein said chelating agent is a chelator of heavy metal ions.

38. A method as claimed in claim 37, wherein said chelator of heavy metal ions is EDTA, and wherein said EDTA has a concentration of 1 mM.

39. A method as claimed in claim 1, wherein said alkyl sulfonate is heptanesulfonate, and wherein said heptanesulfonate has a concentration of about 5 mM.

40. A method as claimed in claim 1, wherein flow of said mobile phase through said apparatus is pulseless.

41. A method as claimed in claim 1, wherein said apparatus is electrically isolated.

42. A method as claimed in claim 41, wherein said pump, said injector and said detector are grounded.

43. A method as claimed in claim 41, wherein said injector, said column and said detector are contained in a Faraday cage.

44. A method as claimed in claim 1, wherein said electrochemical detector cell includes an electrically conductive cap.

45. A method as claimed in claim 1, wherein said electrochemical detector includes electronic controls which are modified to reduce fundamental and non-fundamental noise, and wherein said reduction of fundamental and non-fundamental noise permits the electrochemical detector to maintain a level of sensitivity sufficient to measure the quantity of adrenaline in resting, human plasma.

46. A method as claimed in claim 45, wherein said level of sensitivity of the electrochemical detector is at least one nanoamp full scale deflection.

47. A method as claimed in claim 1, including the steps of monitoring said signal to noise ratio and maintaining said signal to noise ratio above a pre-determined level by maintaining the functional integrity of the electrodes of the electrochemical detector, said electrodes including a glassy carbon working electrode and a stable (gel-filled) reference electrode.

48. A method as claimed in claim 47, wherein said predetermined level of signal to noise ratio is at least about 2 to 1.

49. A method as claimed in claim 47, wherein said predetermined level of signal to noise ratio is at least about 4 to 1.

50. A method as claimed in claim 47, wherein said predetermined level of signal to noise ratio is at least about 8 to 1.

51. A method as claimed in claim 47, wherein the functional integrity of said glassy carbon working electrode is maintained by polishing the working electrode with a polishing medium or compound having a hardness of at least 9 on the Mohs scale, and wherein said polishing step takes place only when the HPLC-ECD apparatus has substantially pure solvent as the mobile phase.

52. A method as claimed in claim 51, wherein said polishing step includes:

(a) exposing said working electrode to polishing compound in the form of an aqueous phase for a period of time ranging from about 30 seconds to about 2 minutes,

(b) removing said polishing compound by the action of water, and

(c) subjecting the polished electrode to sonication to further remove particles of the polishing compound.

53. A method as claimed in claim 52, wherein steps (a), (b) and (c) of the polishing step are performed sequentially with a polishing compound having polishing fragments of about 6 μ

size and a polishing compound having polishing fragments of about 0.25 μ

.

54. A method as claimed in claim 53, wherein said polishing compound comprises natural diamond fragments.

55. A method as claimed in claim 53, wherein said electrode is subjected to said sonication treatment in a solution comprising a mixture of methanol and water, and wherein said methanol has a level of impurity no greater than 10 parts per million, and wherein said water comprises Type I Reagent Grade Water with a minimum resistivity of 10 MΩ

.

56. A method as claimed in claim 1, wherein said step of integrating the current output from said electrochemical detector is performed on an integrator having a means for subtracting a baseline noise component produced by said mobile phase.

59. A method as claimed in claim 1, wherein a plurality of samples are injected sequentially into said high pressure liquid chromatography apparatus and wherein said sensitivity is maintained throughout for assaying of said plurality of samples.

57. A method for the assaying of low concentrations of an oxidizable or reducible compound comprising the steps of:

(a) preparing a sample of at least one of said compounds, said sample being suitable for quantitative analysis;

(b) injecting said sample into a high pressure liquid chromatography apparatus to thereby form a chromatogram, said apparatus including;

(1) a chromatography column containing a quantity of microparticulate material as a stationary phase;

(2) a mobile phase passing through said stationary phase, said mobile phase comprising the following components;

(a) from about 50 mM to about 250 mM of a buffer having a pH of from about 2 to about 8;

(b) from about 0.1 mM to about 10.0 mM of a chelating agent;

(c) from about 0.1 mM to about 10.0 mM of an alkyl sulfonate, said alkyl sulfonate having from about 4 to about 14 carbon atoms; and

(d) from about 0.1% to about 10% methanol in water solution;

(3) an injector for injecting said sample;

(4) a pump for pumping said mobile phase through said stationary phase; and

(5) a guard column positioned upstream of said chromatography column;

(c) eluting said compounds from said chromatography column;

(d) passing said eluted compounds through an electrochemical detector cell thereby producing a current output from said detector;

(e) measuring and integrating the current output from said electrochemical detector to obtain a quantitative analysis of said compound; and

(f) maintaining a sensitivity of said electrochemical detector cell at about 1 nanoamp or less full scale deflection by maintaining all portions of said apparatus contacting said mobile phase and said stationary phase substantially residue-free, wherein the signal to noise ratio of said current output is at least about 2 to 1.

58. A method as claimed in claim 57, wherein the signal to noise ratio of said current output is at least about 8 to 1.

60. A method as claimed in claim 57, wherein a plurality of samples are injected sequentially into said high pressure liquid chromatography apparatus and wherein said sensitivity is maintained throughout for assaying of said plurality of samples.