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DNA detection method

  • US 5,011,770 A
  • Filed: 07/24/1990
  • Issued: 04/30/1991
  • Est. Priority Date: 09/04/1987
  • Status: Expired due to Term
First Claim
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1. A method for quantitatively detecting ssDNA in a sample, said method comprising:

  • combining, in an assay medium, said sample and assay components for detecting said ssDNA, said components comprising;

    a high affinity single-tranded DNA binding protein labeled with a detectable label (d1-BP), a high affinity single-stranded DNA binding protein labeled with a specific binding pair member (BP-sbpm) and an immobilized complementary specific binding pair member complementary to said sbpm-BP (s'"'"'bpm), whereby a DNA complex forms between any ssDNA in said sample and said binding proteins, and said BP-sbpm binds to said immobilized s'"'"'bpm; and

    detecting immobilized detectable label as indicative of the presence of ssDNA in said sample.

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