Integral multilayer analytical element for analysis of albumin
First Claim
1. In an integral multilayer analytical element for the analysis of albumin in a sample containing interfering protein having, in this order, a porous spreading layer, at least one hydrophilic polymer binder layer, and a light-transmissive, water-impermeable support layer, wherein one surface of this spreading layer faces the support layer and the other surface of the spreading layer faces away from the support layer and the support layer contains an indicator compound capable of binding to albumin and the interfering protein to produce a color change,the improvement which comprises said spreading layer containing a pH buffer and said indicator in the spreading layer being dispersed in a hydrophilic polymer or a mixture of a hydrophilic polymer and a hydrophobic polymer, such that the color change due to albumin binding occurs substantially towards the surface of the spreading layer facing the support layer and the color change due to the interfering protein occurs substantially towards the surface facing away from the support layer.
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Abstract
In an integral multilayer analytical element for the analysis of albumin where at least one hydrophilic polymer binder layer and a porous spreading layer containing an indicator capable of binding to albumin to produce a color change are integrally superposed on a light-transmissive water-impermeable support in this order, the improvement comprising said indicator being dispersed in a hydrophilic polymer or a mixture of a hydrophilic polymer and a hydrophobic polymer and a pH buffer being incorporated into the spreading layer.
In the analytical element of the invention, the error caused by the competitive inhibition by globulin can be removed by making to produce the color due to the bonding between albumin and indicator relatively much in the support side (lower side) portion of the porous spreading layer and by making to produce the color due to the bonding between globulin or other proteins and indicator relatively much in the outer surface side (opposite side to the support, upper side) portion of the porous spreading layer.
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7 Claims
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1. In an integral multilayer analytical element for the analysis of albumin in a sample containing interfering protein having, in this order, a porous spreading layer, at least one hydrophilic polymer binder layer, and a light-transmissive, water-impermeable support layer, wherein one surface of this spreading layer faces the support layer and the other surface of the spreading layer faces away from the support layer and the support layer contains an indicator compound capable of binding to albumin and the interfering protein to produce a color change,
the improvement which comprises said spreading layer containing a pH buffer and said indicator in the spreading layer being dispersed in a hydrophilic polymer or a mixture of a hydrophilic polymer and a hydrophobic polymer, such that the color change due to albumin binding occurs substantially towards the surface of the spreading layer facing the support layer and the color change due to the interfering protein occurs substantially towards the surface facing away from the support layer.
Specification