DNA sequences encoding osteoinductive products

• US 5,013,649 A
• Filed: 04/08/1988
• Issued: 05/07/1991
• Est. Priority Date: 07/01/1986
• Status: Expired due to Term

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Genetics Institute LLC (Pfizer Inc.)
• Inventors: Wozney, John M., Rosen, Vicki, Wang, Elizabeth A.
• Primary Examiner(s): Teskin, Robin L.
• Assistant Examiner(s): Ellis, Joan
• Application Number:
  US07/179,100
• Time in Patent Office: 1,124 Days
• Field of Search: 435/68, 435/91, 435/172.1, 435/172.3, 435/320, 435/252, 536/27, 530/350, 935/18, 935/22, 935/33, 935/55, 935/60, 935/66, 935/76, 935/69.1, 935/235, 935/240.2, 935/255
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 24/043 Mixtures of macromolecular materials
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00

Abstract

Purified BMP-2 proteins and processes for producing them are disclosed. They may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.

Claims

1. An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising a coding sequence selected from the group consisting of

(a) nucleotide #1 through nucleotide #387 of figure I,

(b) nucleotide #356 through nucleotide #1543 of figure II,

(c) Nucleotide #402 through nucleotide #1626 of figure III,

(d) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a), (b), and (c);

and

(e) sequences which

(1) hybridize to any of sequences (a), (b), (c), or (d) under stringent hybridization conditions; and

(2) encode a protein characterized by the ability to induce the formation of bone and/or cartilage.

2. A vector comprising a DNA sequence of claim 1 in operative association with an expression control sequence for said DNA sequence.

3. A host cell transformed with the vector of claim 2 said host cells selected from the group consisting of mammalian, bacterial, yeast and insect cells.

4. A method for producing an osteoinductive protein, said method comprising the steps of

(a) culturing in a suitable culture medium said transformed host cell of claim 3;

and

(b) isolating and purifying said osteoinductive protein from said culture medium.

5. The host cell of claim 4 wherein said host cell is a mammalian cell.

6. The host cell of claim 4 wherein said cells are selected from the group consisting of bacterial, yeast and insect cells.

7. A mammalian host cell transformed with the vector of claim 2.