Molecular diagnosis of Alzheimer Disease
First Claim
1. A method for diagnosing Alzheimer Disease or a predisposition to develop Alzheimer Disease in an individual, comprising the step of determining the presence of an alteration in the normal DNA base sequence
or its normal complementary DNA or RNA sequences, in a sample of the DNA or RNA of such individual by hybridization with an aligonucleotide probe.
1 Assignment
0 Petitions
Accused Products
Abstract
A method for diagnosing Alzheimer Disease or a predisposition to develop Alzheimer Disease in an individual, by determining the presence of an alteration in the normal base sequence of the 24 base pairs appearing at the end of the APC coding sequence of the preAPC gene, or in the normal base sequence of the corresponding mRNA, in the DNA or RNA or such individual. The method detects the presence of a frameshift mutation in the preAPC gene postulated to result in the synthesis of the APC protein. Accumulation of APC protein in the brain is a phenomenon of Alzheimer Disease. A variety of nucleic acid hybridization techniques are applicable to the determination of an alteration in the preAPC gene sequence of interest.
-
Citations
20 Claims
- 1. A method for diagnosing Alzheimer Disease or a predisposition to develop Alzheimer Disease in an individual, comprising the step of determining the presence of an alteration in the normal DNA base sequence
- space="preserve" listing-type="equation">'"'"' CAC TGT CGC TAT GAC AAC ACC GCC 3'"'"',
or its normal complementary DNA or RNA sequences, in a sample of the DNA or RNA of such individual by hybridization with an aligonucleotide probe. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
- 4. The method of claim 3 wherein said oligonucleotide probe consists of less than 50 nucleotides and includes the 24 nucleotide DNA base sequence
- space="preserve" listing-type="equation">5'"'"' CAC TGT CGC TAT GAC AAC ACC GCC 3'"'"',
or its corresponding RNA base sequence, or their complementary DNA or RNA sequences.
- 12. An oligonucleotide probe for use in the diagnosis of Alzheimer Disease or a predisposition to develop Alzheimer Disease, consisting essentially of less than 50 nucleotides and including the 24 nucleotide DNA base sequence
- space="preserve" listing-type="equation">5'"'"' CAC TGT CGC TAT GAC AAC ACC GCC 3'"'"'
or its corresponding RNA base sequence, or their complementary DNA or RNA sequences. - View Dependent Claims (13, 14, 15, 16)
- 17. An oligonucleotide probe for use in the diagnosis of Alzheimer Disease or a predisposition to develop Alzheimer Disease, consisting essentially of at least 10 nucleotides whose base sequence is homologous with the DNA sequence of the preAPC gene having a mutation in the 24 nucleotide sequence
- space="preserve" listing-type="equation">5'"'"' CAC TGT CGC TAT GAC AAC ACC GCC 3'"'"',
or in its complementary DNA or RNA sequence. - View Dependent Claims (18, 19, 20)
Specification