Device for immunoassay determinations

• US 5,017,342 A
• Filed: 06/01/1988
• Issued: 05/21/1991
• Est. Priority Date: 06/01/1988
• Status: Expired due to Term

• Current Assignee: Meridian Bioscience, Inc.
• Original Assignee: Ortho Diagnostic Systems, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Inventors: Geltosky, Jack, Perst, Renee, Haberzettl, Cecelia
• Primary Examiner(s): Lacey, David L.
• Assistant Examiner(s): DALEY, THOMAS
• Application Number:
  US07/201,515
• Time in Patent Office: 1,084 Days
• Field of Search: 436/535, 436/810, 436/809, 435/310, 435/299, 422/297, 422/301, 422/55, 422/58, 422/102, 422/99, 220/23.8, 294/7, 294/30, 294/55, 30/324, 30/141, 30/149, 30/325, 30/326, 30/327, 30/328
• US Class Current: 422/552
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

An assay device is provided which includes a support, generally planar and elongated in shape, upon which a biological binding reaction may take place. This assay support includes in its broadest aspect, three sections, a sample receiving well section at one end and a gripping section at the opposite end, with a connecting section therebetween. The sample receiving well section includes at least two spaced-apart receiving wells, generally at the end of the assay support, for the addition of binding reactants. Each of the wells has an open end on a first face of the support, a concave inner surface extending concavely into the support, and a convex outer surface which projects convexly from an opposed face of the support. These wells may serve as a depository to contain aliquots of binding reagents, as well as a sample under analysis, the sample under analysis possibly containing a substance that will bind to the binding reagents.

Claims

1. An assay support, generally planar and elongated in shape, and comprising a gripping section, a sample receiving well section, and a connecting section therebetween, each of said sections being aligned along a longitudinal axis;

said sample receiving well section comprising at least two spaced-apart receiving wells;

each of said wells having an open end on a first face of said support, a concave inner surface extending concavely into said support, and a convex outer surface which projects convexly from an opposed face of said support;

said convex outer surface of at least one of said wells provided with at least one stabilizing leg constructed and arranged to preclude rotation of said wells when resting on a flat surface;

said assay support provided with a leveling projection extending from said opposed face so as to cooperate with said at least one stabilizing leg to maintain said support level when resting on a flat surface;

said sample receiving section in the space between said wells comprises a dam means projecting from the first face of said support so as to preclude the flow of a liquid sample from one of said wells to another of said wells.

2. The assay support of claim 1 wherein said concave inner surface of said wells further comprises ridges, adapted to aid in the homogenous dispersing of liquid reactants deposited therein.

3. The assay support of claim 2 wherein said ridges are in the form of concentric circles.

4. The assay support of claim 1 wherein said dam means further comprises at least one labeling portion.

5. The assay support of claim 4 wherein said at least one labeling portion is contiguous with said dam means, and projects from said first face of said planar support to a height equal to that of said dam means.

6. The assay support of claim 5 wherein said at least one labeling portion is generally triangular in shape with an apex extending toward one of said receiving wells.

7. The assay support of claim 6 wherein said at least one labeling portion further comprises labeling indicia.

8. The assay support of claim 7 wherein within said connecting section of said assay support includes a labeling region for the labeling of said assay support.

9. The assay support of claim 8 wherein said labeling region is contiguous with said gripping section of said assay support.

10. The assay support of claim 9 wherein said leveling projection, adapted to cooperate with at least one stabilizing leg, is positioned between said labeling region of said connecting section and said gripping section.

11. The assay support of claim 10 wherein said gripping section comprises ridges to facilitate the manual gripping of said support.

12. The assay support of claim 11 wherein said gripping section further comprises numbering indicia.

13. A plurality of detachably connected assay supports wherein each of said assay supports is generally planar and elongated in shape and comprises a gripping section, a sample receiving well section and a connecting section therebetween, each of said sections being aligned along a longitudinal axis;

said sample receiving well section comprising at least two spaced-apart receiving wells;

each of said wells having an open end on a first face of said support, a concave inner surface extending concavely into said support, and a convex outer surface which projects convexly from an opposed face of said support;

said convex surface of at least one of said wells provided with at least one stabilizing leg constructed and arranged so as to preclude rotation of said wells when resting on a flat surface;

said assay support provided with a leveling projection extending from said opposed face so as to cooperate with said at least one stabilizing leg to maintain said support level when resting on a flat surface;

said sample receiving well section in the space between said wells comprises dam means projecting from the first face of said planar support, so as to preclude the flow of a liquid sample from one of said wells to another of said wells.

14. The plurality of assay supports of claim 13 wherein said assay supports are detachably connected one to another generally at said gripping section of each of said supports.

15. The detachably connected assay supports of claim 14, wherein said supports are connected in a substantially single plane to form a planar series of said supports for applying reagents to said wells by dipping said wells into a bath together with a plurality of similar planar series, so that the first face of the assay supports of said series faces the opposing face of an adjacent planar series;

said assay supports each including a spacer projection extending from the sample receiving section of the opposed face so that it will cooperate with the dam means of the assay support of an adjacent planar series of said assay supports to keep the convex surface of the wells of said planar series apart from the concave surface of the wells of the adjacent planar series of assay supports.

16. The plurality of assay supports of claim 15 wherein said plurality of assay supports comprises about 8 to about 10 of said detachably connected assay supports.

17. An assay support, useful in conducting immunoassay determinations of the ratio of IgG to IgM antibodies in biological samples, said support generally planar and elongated in shape, and comprising a gripping section, a sample receiving well section, and a connecting section therebetween, each of said sections being aligned along a longitudinal axis;

said sample receiving well section comprising two spaced-apart receiving wells;

each of said wells having an open end on a first face of said support, a concave inner surface extending concavely into said support, said inner surface comprising concentric circles; and

a convex outer surface which projects convexly from an opposed face of said support;

said convex outer surface of at least one of said wells includes at least one stabilizing leg adapted to preclude rotation of said wells when resting on a flat surface;

said assay support comprises a leveling projection extending from said opposed face so as to cooperate with said at least one stabilizing leg to maintain said support level when resting on a flat surface;

said sample receiving well section also comprising in the space between said wells dam means projecting from the first face of said support so as to preclude the flow of a liquid sample from one of said wells to another of said wells.

said dam means further comprising a labeling portion contiguous therewith and projecting from said first face of said support to a height equal to that of the dam means;

said labeling portion being generally triangular in shape with an apex extending toward one of said receiving wells.

18. The device of claim 17, wherein said receiving well section is pre-coated with a protein capable of binding to an antibody.