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Homogeneous chemiluminescence immunoassay using a light absorbing material

  • US 5,017,473 A
  • Filed: 05/26/1987
  • Issued: 05/21/1991
  • Est. Priority Date: 05/26/1987
  • Status: Expired due to Fees
First Claim
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1. A method for determining an unknown quantity of an analyte in a fluid comprising:

  • (a) preparing a mixture comprising an antianalyte attached to the surface of a tube, an analyte in a fluid, a light absorbing material, and a tracer for said analyte, said tracer including a chemiluminescent label;

    (b) causing binding involving immunologically specific pairs of said antianalyte, said analyte and said tracer whereby bound tracer is formed on the surface of said tube;

    (c) adding to said mixture a composite comprising an activator of a chemiluminescence source whereby chemiluminescence is induced;

    (d) measuring chemiluminescence associated with said bound tracer; and

    (e) determining the quantity of said analyte in said fluid by comparing the magnitude of said chemiluminescence with the magnitude of chemiluminescence established for a known quantity of the analyte.

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