×

Method and apparatus for performing high sensitivity fluorescence measurements

  • US 5,018,866 A
  • Filed: 09/12/1989
  • Issued: 05/28/1991
  • Est. Priority Date: 09/12/1989
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method of performing fluorescence measurements on a sample capable of being activated by exciting radiation, said method comprising the steps of:

  • enclosing the sample within a container havingsurrounding walls and an open side facing a bottom surface, said bottom surface having reflector means for reflecting any radiation incident thereupon;

    introducing a beam of exciting radiation through said open side of container directly onto the sample contained therein in a direction normal to said reflector surface in such a way that said beam does not reach said container walls, thereby avoiding any secondary fluorescence therefrom, any portion of said beam which is unabsorbed by the sample and traverses through the sample to strike said reflector means on si bottom surface being reflected therefrom back through the sample without reaching said container walls, thereby avoiding any secondary fluorescence therefrom;

    collecting fluorescent radiation from the sample through collector means surrounding said container walls, said collector means being adapted to focus said fluorescent radiation incident thereupon onto a predefined area; and

    detecting and measuring said fluorescent radiation focused onto said predefined area.

View all claims
  • 4 Assignments
Timeline View
Assignment View
    ×
    ×