Sex-associated membrane antibodies and their use for increasing the probability that offspring will be of a desired sex

• US 5,021,244 A
• Filed: 05/12/1989
• Issued: 06/04/1991
• Est. Priority Date: 12/06/1988
• Status: Expired due to Fees

• Current Assignee: Cytogam Incorporated
• Original Assignee: Cytogam Incorporated
• Inventors: Spaulding, Glenn F.
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US07/351,642
• Time in Patent Office: 753 Days
• Field of Search: 435/2, 424/105, 424/561
• US Class Current: 530/388.2
• CPC Class Codes: A61K 35/52 Sperm; Prostate; Seminal fluid; Leydig cells of testes
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: G01N 2800/367 Infertility, e.g. sperm disorder, ovulatory dysfunction
• CPC Class Codes: Y10S 424/811 involving sex selection or contraception

Abstract

A method of sorting living cells based on DNA content. Mammalian sperm subpopulations enriched in X- or Y-sperm. X- and Y-enriched sperm-plasma-membrane vesicles. Substantially pure sex-associated membrane (SAM) proteins. Antibodies binding to X- or Y-SAM proteins, essentially free of antibodies binding to Y- or X-SAM proteins respectively, or to the H-Y antigen. Semen samples enriched for X- or Y-sperm. Methods for increasing the probability that offspring will be male or female comprising the step of allowing as perm from an enriched semen sample to fertilize an ovum. Methods for increasing the probability that offspring will be male or female comprising the step of immunizing a female with X- or Y-SAM proteins. Methods of decreasing fertility comprising the step of immunizing a female with both X- and Y-sperm. Methods of increasing the probability that mammalian offspring will carry a gene for a particular sex-chromosome linked trait.

Claims

1. An antibody which binds to a mammalian X sex-associated membrane (X-SAM) protein, said antibody being essentially free of antibodies which bind to a mammalian Y sex-associated membrane protein, to the H-Y antigen, or to non-sex-specific components of the sperm plasma membrane.

3. The antibody of claim 1 or 2 wherein said antibody is a monoclonal antibody.

4. The antibody of claim 1 or 2 wherein said X-SAM protein is from cattle.

5. The antibody of claim 1 or 2 wherein said X-SAM protein is from a species selected from the group consisting of dog, cat, horse, pig, or sheep.

6. The antibody of claim 1 or 2 wherein said X-SAM protein is from humans.

7. A mammalian semen sample enriched for Y-sperm comprising viable Y-sperm and X-sperm substantially inactivated with the antibody of claim 1.

9. The semen sample of claim 7 or 8 wherein the inactivating conditions include incubation in phosphate buffer, pH 6.4-pH 8.4 at 37°

C. for at least 15 min with an excess of antibody.

10. A method for increasing the probability that a mammalian offspring will be male comprising that step of allowing a sperm from the semen sample of claim 7 to fertilize an ovum.

12. The method of any one of claims 10-11 wherein the mammal is a cattle specie.

13. The method of any one of claims 10-11 wherein the mammal is selected from the group consisting of dog, cat, horse, sheep, and pig.

14. The method of any one of claims 10-11 wherein the mammal is a human.

15. An artifical insemination kit comprising a straw containing the semen sample of claim 7 and a cryopreservative.

17. The kit of claim 15 or 16 wherein the semen sample is from cattle.

18. A method of increasing the probability that a mammalian offspring will carry a gene for a particular Y-chromosome linked trait or decreasing the probability that a mammalian offspring will carry a gene for a particular X-chromsome-linked trait comprising the step of allowing a sperm from the semen sample of claim 7 to fertilize an ovum.

2. An antibody which binds to a mammalian Y sex-associated membrane (Y-SAM) protein, said antibody being essentially free of antibodies which bind to a mammalian X sex-associated membrane protein, to the H-Y antigen, or to non-sex-specific components of the sperm plasma membrane.

8. A mammalian semen sample enriched for X-sperm comprising viable X-sperm and Y-sperm substantially inactivated with the antibody of claim 2.

11. A method for increasing the probability that a mammalian offspring will be female comprising the step of allowing a sperm from the semen sample of claim 8 to fertilize an ovum.

16. An artifical insemination kit comprising a straw containing the semen sample of claim 8 and a cryopreservative.

19. A method of increasing the probability that a mammalian offspring will carry a gene for a particular X-chromosome linked trait or decreasing the probability that a mammalian offspring will carry a gene for a particular Y-chromsome-linked trait comprising the step of allowing a sperm from the semen sample of claim 8 to fertilize an ovum.