Remote optical path for capillary electrophoresis instrument

• US 5,021,646 A
• Filed: 01/25/1990
• Issued: 06/04/1991
• Est. Priority Date: 01/25/1990
• Status: Expired due to Term

• Current Assignee: Thermo BioAnalysis Corp. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Spectra-Physics Incorporated (MKS Instruments Incorporated)
• Inventors: Hoppe, Thomas W., Weinberger, Scot R.
• Primary Examiner(s): Nelms, David C.
• Application Number:
  US07/470,287
• Time in Patent Office: 495 Days
• Field of Search: 250/327.2 C, 250/227.11, 250/227.28, 356/319, 356/320
• US Class Current: 250/227.11
• CPC Class Codes: G01N 27/44708 Cooling
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

An electrophoresis instrument includes a capillary tube mounted in an air cooled cartridge. The cartridge also supports a spherical lens which is part of the optical detection apparatus. The cartridge rests in a manifold which includes the sample and buffer reservoirs. The temperature of the capillary tube is controlled by measuring the electrical resistance of the capillary tube during the electrophoresis process and then cooling or heating the cartridge by circulating temperature controlled air over the tube. The optical path associated with the instrument is a fiber optic bundle bifurcated close to dual detectors into a reference arm and a sample arm so as to provide similar reference and sample optical paths. The instrument may be used for controlling temperature in gradient electrophoresis and detecting neutral markers in determining electro-osmotic flow.

Claims

1. An optical path for an optical instrument comprising:

a light source;

diffraction means for diffracting light from the light source;

a beam splitter including an entry end for admitting light from the diffraction means and including a plurality of optical fibers leading from the entry end to an exit end of the beam splitter;

a sample detector and a reference detector both located adjacent to the exit end of the beam splitter;

wherein a first portion of the optical fibers in the beam splitter transmits light from the entry end of the beam splitter to the sample detector and a second portion of the optical fibers transmits light from the entrance end of the beam splitter to the reference detector, wherein the optical fibers are bifurcated amongst the first and second portions at a point adjacent to the reference detector.

2. The device of claim 1, wherein the optical fibers are grouped into groups of three fibers, wherein two of the fibers in each group transmit light to the sample detector and wherein one of the fibers in each group transmits light to the reference detector.

3. The device of claim 1, wherein a cross sectional shape of the optical fibers in the beam splitter is a circle.

4. The device of claim 1, wherein a cross sectional shape of the optical fibers in the beam splitter is a parallelogram.

5. The device of claim 1, wherein the beam splitter is housed in a retractable member.