Area-modulated luminescence (AML)
First Claim
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1. A device comprising:
- an excitation optical system projecting excitation light of a constant radiant power or photon flux at a wavelength or wavelengths selected for a sample into said sample;
a sample cell for said sample;
means for alternately irradiating different regions of said sample having different volumes;
emitted light sensing means sensing the emitted light;
means for comparing the difference in the emitted light from said regions of different volumes.
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Abstract
A luminescence measuring system is provided for detecting luminescence at extremely low concentrations of luminescing moieties. The method employs alternating radiation at a plurality of loci of an inhomogenous solution, where the radiant power is maintained constant, and the irradiated volumes of pairs of loci are systematically varied. With the probability being very low that the same luminescence signal will be obtained in the two or more measurements, by comparing the measurements, one can detect a low luminescence signal in the presence of relatively high noise levels. Various techniques are described for modulating the irradiance and detecting changes in signal.
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6 Claims
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1. A device comprising:
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an excitation optical system projecting excitation light of a constant radiant power or photon flux at a wavelength or wavelengths selected for a sample into said sample; a sample cell for said sample; means for alternately irradiating different regions of said sample having different volumes; emitted light sensing means sensing the emitted light; means for comparing the difference in the emitted light from said regions of different volumes. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification