RNA polymerase gene, microorganism having said gene and the production of RNA polymerase by the use of said microorganism

• US 5,026,645 A
• Filed: 10/19/1987
• Issued: 06/25/1991
• Est. Priority Date: 10/21/1986
• Status: Expired due to Term

• Current Assignee: Brookhaven Science Associates, LLC (Government of the United States of America)
• Original Assignee: Takara Shuzo Company Limited (Ningbo Junsheng Investment Group Co., Ltd.)
• Inventors: Hiraoka, Nobutsugu, Obayashi, Akira, Kotani, Hirokazu
• Primary Examiner(s): Schwartz, Richard A.
• Assistant Examiner(s): NOLAN, SHARON
• Application Number:
  US07/109,615
• Time in Patent Office: 1,345 Days
• Field of Search: 435/235, 435/194, 435/252.3, 435/252.33, 435/320, 536/27, 932/14, 932/29, 932/27, 932/73
• US Class Current: 435/194
• CPC Class Codes: C12N 9/1247 DNA-directed RNA polymerase (2.7.7.6)

Abstract

SP6 bacteriophage RNA polymerase is produced by cultivating a new microorganism (particularly new strains of Escherichia coli) harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene and recovering SP6 bacteriophage RNA polymerase from the culture broth. SP6 bacteriophage RNA polymerase gene is provided as are new microorganisms harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene.

Claims

1. An isolated gene encoding SP6 bacteriophage RNA polymerase.

2. Escherichia coli containing a plasmid which comprises an isolated gene encoding SP6 bacteriophage RNA polymerase.

3. Process for producing SP6 bacteriophage RNA polymerase which comprises cultivating Escherichia coli containing a plasmid that carries an isolated SP6 bacteriophage RNA polymerase gene under conditions sufficient to produce the SP6 bacteriophage RNA polymerase and recovering the SP6 bacteriophage RNA polymerase from the culture.