Apparatus for determination of probe contact with a liquid surface

• US 5,027,075 A
• Filed: 09/18/1989
• Issued: 06/25/1991
• Est. Priority Date: 09/18/1989
• Status: Expired due to Fees

• Current Assignee: Nova Biomedical Corporation (Nova Chemicals)
• Original Assignee: Nova Biomedical Corporation (Nova Chemicals)
• Inventors: Harding, John C. Jr.
• Primary Examiner(s): Wieder, Kenneth
• Assistant Examiner(s): Harvey, Jack B.
• Application Number:
  US07/408,616
• Time in Patent Office: 645 Days
• Field of Search: 324/661, 324/662, 324/664, 324/676, 324/689, 324/71.1, 340/620, 73/864.24, 73/864.25, 73/304 C, 422/63, 422/67, 422/100, 422/106
• US Class Current: 324/662
• CPC Class Codes: G01F 23/245 with a probe moved by an auxiliary power, e.g. meter, to follow automatically the level
• CPC Class Codes: G01F 23/263 by measuring variations in capacitance of capacitors
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 2035/1076 plurality or independently movable heads
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/1072 with provision for selective pipetting of individual channels
• CPC Class Codes: G05D 9/12 characterised by the use of electric means

Abstract

The occurrence of physical contact between a conductive probe and a surface of a liquid held in a container is detected as the probe is moved toward and eventually touches the liquid surface; a conductor is disposed on the other side of the container from the probe; an electrical signal is sent through the liquid between the conductive probe and the conductor, and the time when physical contact has occurred is determined based on when the resulting electrical signal which has passed through the liquid exceeds a threshold; and the threshold is determined based on the resulting electrical signal. In another aspect, the electrical signal is pulsed, and the resulting electrical signal is synchronously detected as the basis for determining when the physical contact has occurred. In another aspect, a non-conductive rotatable support holds the container and moves the container into position under the probe; a conductor is disposed beneath the rotatable support and in a fixed position relative to the rotatable support so that when the container is in position under the probe, the conductor lies beneath the container; the conductor has a limited extent so that when the container is not in that position no part of the conductor lies beneath the container.

Claims

1. Apparatus for detecting the occurrence of physical contact between a conductive probe and a surface of a liquid held in a container as the probe is moved toward and eventually touches the liquid surface, the apparatus comprising:

a conductor disposed on the other side of said container from said probe,

signaling circuitry for sending an electrical signal through said liquid between said conductive probe and said conductor, and for detecting when said physical contact has occurred based on when the resulting electrical signal which has passed through said liquid exceeds a threshold, and

a threshold determiner for establishing said threshold based on said resulting electrical signal.

2. The apparatus of claim 1 wherein said threshold determiner is arranged to establish said threshold based on said resulting electrical signal prior to said probe touching said liquid surface.

3. The apparatus of claim 1 wherein said threshold determiner is connected to receive two values of said resulting electrical signal with said probe positioned at two distinct points above said surface and said threshold determiner is arranged to calculate said threshold value based on both said received values.

4. The apparatus of claim 1 wherein said threshold determiner is further arranged to calculate the expected value that said resulting electrical signal would have if the probe were positioned at a predetermined point within said container, and to set said threshold value to a predetermined amount above said expected value.

5. Apparatus for detecting the occurrence of physical contact between a conductive probe and a surface of a liquid held in a container as the probe is moved toward and eventually touches the liquid surface, the apparatus comprising:

a conductor disposed on the other side of said container from said probe; and

circuitry for sending a pulsed electrical signal through said liquid between said conductive probe and said conductor while said probe is being moved, and for synchronously detecting the resulting electrical signal as the basis for determining when said physical contact has occurred.

6. The apparatus of claim 5 wherein said circuitry comprises a pulse generator for supplying said electrical signal.

7. The apparatus of claim 6 wherein said circuitry comprises a synchronous detector for detecting said resulting electrical signal, and said pulse generator is connected to said synchronous detector.

8. The apparatus of claim 5 wherein said circuitry determines when said physical contact has occurred by detecting a step change in said resulting electrical signal.

9. The apparatus of claim 5 wherein said circuitry detects when said physical contact has occurred by detecting when said resulting electrical signal exceeds a threshold.

10. The apparatus of claim 9 further comprising a threshold determiner for receiving values of said resulting electrical signal and calculating said threshold based on said received values.

11. The apparatus of claim 10 wherein said threshold is determined based on the value of said resulting electrical signal with said probe positioned at two distinct points above said surface.

12. The apparatus of claim 10 wherein said threshold determiner is further arranged to calculate the expected value that said resulting electrical signal would have if the probe were positioned at a predetermined point within said container, and to set said threshold value to a predetermined amount above said expected value.

13. A method for detecting the occurrence of physical contact between a conductive probe and a surface of a liquid held in a container as the probe is moved toward and eventually touches the liquid surface, the method comprising:

sending an electrical signal through said liquid between said conductive probe and a conductor disposed on the other side of said container from said probe while said probe is being moved,

determining a threshold based on the resulting electrical signal which has passed through said liquid, and

detecting when said physical contact has occurred based on when said resulting electrical signal exceeds said threshold.

14. The method of claim 13 wherein said step of determining comprises measuring the value of said resulting electrical signal with said probe positioned at two distinct points above said liquid surface.

15. The method of claim 14 wherein said step of determining comprises calculating the expected value that said resulting electrical signal would have if the probe were positioned at a predetermined point within said container, and setting said threshold value to a predetermined amount above said expected value.

16. The method of claim 15 wherein said expected value is calculated based on values of said resulting electrical signal with said probe positioned above said liquid surface.

17. A method for detecting the occurrence of physical contact between a conductive probe and a surface of a liquid held in a container as the probe is moved toward and eventually touches the liquid surface, the method comprising:

sending a pulsed electrical signal through said liquid between said conductive probe and a conductor disposed on the other side of said container from said probe, and

synchronously detecting the resulting electrical signal as the basis for determining when said physical contact has occurred.

18. The method of claim 17 wherein said step of synchronously detecting comprises detecting a step change in said resulting electrical signal.

19. The method of claim 17 wherein said step of synchronously detecting comprises detecting when said resulting electrical signal exceeds a threshold.

20. Apparatus for detecting the occurrence of physical contact between a conductive probe and a surface of a liquid held in a container as the probe is moved toward and eventually touches the liquid surface, the apparatus comprising:

a non-conductive rotatable support for holding said container and moving said container into position under said probe,

a conductor disposed beneath said rotatable support and in a fixed position relative to said rotatable support so that when said container is in said position said conductor lies beneath said container and on the other side thereof from said probe, said conductor having a limited extend so that when said container is not in said position no part of said conductor lies beneath said container, and

circuitry for sending a pulsed electrical signal through said liquid between said conductive probe and said conductor while said probe is being moved, and for synchronously detecting the resulting electrical signal as the basis for determining when said physical contact has occurred.

21. The apparatus of claim 20 wherein said conductor comprises a plate.

22. The apparatus of claim 21 wherein said plate is L-shaped and extends both beneath and adjacent said rotatable support.

23. The apparatus of claim 20 wherein said rotatable support comprises a tray which supports a plurality of containers, each of which may be positioned beneath said probe by rotation of said tray.