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Threshold ligand-receptor assay

  • US 5,028,535 A
  • Filed: 01/10/1989
  • Issued: 07/02/1991
  • Est. Priority Date: 01/10/1989
  • Status: Expired due to Term
First Claim
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1. Method for determining the presence of at least one target ligand, capable of competing with a ligand analogue conjugate for binding sites available on a ligand receptor, said ligand analogue conjugate comprising at least one ligand analogue coupled to a signal development element capable of emitting a detectable signal, in a fluid sample suspected of containing said target ligand, comprising the steps of:

  • a. contacting said fluid sample with said ligand analogue conjugate and said ligand receptor to form a homogeneous reaction mixture, the relative amounts of said ligand analogue conjugate and said ligand receptor being selected such that in the absence of said target ligand, and subsequent to substantially equilibrium binding in said reaction mixture, substantially all of said ligand analogue conjugate is bound to said ligand receptor such that no unbound ligand analogue conjugant is detected as a result of the assay method;

    b. detecting unbound ligand analogue conjugate in said reaction mixture;

    c. relating the detectable signal to the presence of said target ligand in said fluid sample.

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