Ethanol production using engineered mutant E. coli
First Claim
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1. A mutant Escherichia coli strain with a gene mutation, which results in hyperproduction of alcohol dehydrogenase which has been transformed with a Zymomonas mobilis gene coding for pyruvate decarboxylase, wherein said pyruvate decarboxylase gene is expressed at sufficient levels to confer upon said Escherichia coli transformant the ability to produce ethanol as a fermentation product.
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Abstract
The subject invention concerns novel means and materials for producing ethanol as a fermentation product. Mutant E. coli are transformed with a gene coding for pyruvate decarboxylase activity. The resulting system is capable of producing relatively large amounts of ethanol from a variety of biomass sources.
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4 Claims
- 1. A mutant Escherichia coli strain with a gene mutation, which results in hyperproduction of alcohol dehydrogenase which has been transformed with a Zymomonas mobilis gene coding for pyruvate decarboxylase, wherein said pyruvate decarboxylase gene is expressed at sufficient levels to confer upon said Escherichia coli transformant the ability to produce ethanol as a fermentation product.
- 3. A method for the production of ethanol, said method comprising the growth of a mutant Escherichia coli with a gene mutation which results in hyperproduction of alcohol dehydrogenase which has been transformed with a Zymomonas mobilis gene coding for pyruvate decarboxylase, wherein said pyruvate decarboxylase gene is expressed at sufficient levels to confer upon said Escherichia coli transformant the ability to produce ethanol as a fermentation product.
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Current AssigneeHitachi, Ltd., University of Florida Research Foundation, Incorporated (State University System of Florida)
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Original AssigneeUniversity of Florida (State University System of Florida)
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InventorsClark, David P., Ingram, Lonnie O.
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Primary Examiner(s)Teskin, Robin L.
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Application NumberUS07/274,075Time in Patent Office953 DaysField of Search435/161, 435/170, 435/172.3, 435/320, 536/27, 935/27, 935/42, 935/73US Class Current435/161CPC Class CodesC12N 15/52 Genes encoding for enzymes ...C12P 7/065 with microorganisms other t...G06T 1/00 General purpose image data ...Y02E 50/10 Biofuels, e.g. bio-diesel
