Electrode for electrochemical sensors

• US 5,030,310 A
• Filed: 01/19/1990
• Issued: 07/09/1991
• Est. Priority Date: 06/28/1985
• Status: Expired due to Term

• Current Assignee: Miles, Inc. (Bayer AG)
• Original Assignee: Miles, Inc. (Bayer AG)
• Inventors: Wogoman, Frank W.
• Primary Examiner(s): Weston, Caleb
• Application Number:
  US07/467,363
• Time in Patent Office: 536 Days
• Field of Search: 156/252, 204/403, 204/412, 204/415, 204/416, 204/418, 435/817, 128/635
• US Class Current: 156/252
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode
• CPC Class Codes: Y10T 156/1056 Perforating lamina

Abstract

A disposable, inexpensive electrode for an electrochemical sensor includes a laminated anode and a laminated cathode. The anode is a film substrate with anodic material such as platinum deposited on the substrate. The cathode is also a film substrate with cathodic material such as silver deposited on the substrate. The anode and cathode can be laminated with an intermediate layer of dielectric material. A working surface is provided on the electrode and defined, in part, by the anode and cathode. One procedure for defining the working surface is to fabricate spaced openings in the cathode film and the layer of dielectric material. The openings in the cathode film and dielectrical layer are aligned and the exposed surfaces of the anode and cathode define the working area. An enzyme, such as glucose oxidase, is bonded to the exposed anode and a membrane is applied over the working area and enzyme. The membrane is preferably a silicone water-based elastomer. The laminated anode, cathode and dielectric layer are then severed to form individual electrodes each including a working area.

Claims

1. A method of fabricating an electrode, comprising the steps of:

fabricating an opening in a layer of cathodic material;

fabricating an opening in a layer of dielectric material;

laminating said layer of cathodic material to one side of said layer of dielectric material and laminating a layer of anodic material to the opposite side of said layer of dielectric material such that the opening in the layer of dielectric material and the opening in the layer of cathodic material are aligned to form a working area;

inserting glucose oxidase in said working area onto at least a portion of said layer of anodic material; and

applying a glucose permeable, silicon water-based elastomer over said glucose oxidase in the aligned openings forming said working area.

2. The method of claim 1 in which the layer of cathodic material, the layer of dielectric material and the layer of anodic material are severed to form an individual electrode following lamination of said layers.

3. The method of claim 1 in which the glucose permeable, silicon water-based elastomer is cured after being applied over the glucose oxidase in said working area.

4. The method of claim 1 in which the layer of cathodic material and the layer of anodic material are laminated to the layer of dielectric material simultaneously.

5. The method of claim 1 which further includes connecting separate electrical connectors to said layer of cathodic material and to said layer of anodic material.