Integral centrifuge tube and specimen slide

• US 5,030,421 A
• Filed: 08/31/1988
• Issued: 07/09/1991
• Est. Priority Date: 08/31/1988
• Status: Expired due to Fees

• Current Assignee: IRIS International Inc. (Danaher Corporation)
• Original Assignee: Davstar Industries, Inc.
• Inventors: Muller, Louis F.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): KRYZA, LYNN M
• Application Number:
  US07/238,896
• Time in Patent Office: 1,042 Days
• Field of Search: 422/58, 422/61, 422/64, 422/72, 422/102, 436/45, 436/46, 436/165, 436/177, 436/180, 436/808, 436/810, 435/296, 435/310, 494/16, 356/246, 356/244, 356/440, 356/427, 350/442, 350/319
• US Class Current: 422/549
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/112499 with sample on test slide
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A generally tubular container integrally formed and communicating with a microscope slide member having an enclosed specimen chamber. The container is adapted to serve as a centrifugation tube and a permanent handle for manipulation of the microscope slide member. The back of the device is generally flat and the front and sides of the collection tube are generally rounded except for a concave region extending longitudinally from the upper face of the microscope slide member to provide clearance for rotating a lens turret for high power viewing of a specimen in the slide chamber.

Claims

1. An integral specimen collection tube and microscope slide device which comprises in combination:

an elongated tubular container portion having means defining an entrance opening at a proximal end and an exit opening at a distal end, said container portion having a flat planar surface extending along a rear side thereof;

a slide portion joined to the container portion at said distal end and extending longitudinally beyond the container portion to a closed terminal end of the device;

the slide portion comprising flat, optically transparent, opposed parallel base and cover members defining a chamber for receiving a specimen and displaying same for viewing through a microscope having a slide supporting stage;

means defining a transition portion between the tubular container and slide portions for locating the slide portion base member in alignment with said planar surface adjacent the microscope stage when the device is placed on the stage with the flat rear side in contact with the stage; and

means defining a lens clearance section of the tubular container portion in the form of a generally transversely flat, longitudinally curved, concave surface extending from the cover member to a juncture with the wall of the tubular container portion remote from the cover member opposite the longitudinal center line of said planar surface.

2. The device of claim 1 wherein said concave surface extends generally tangentially from the proximal end of the cover member.

3. The device of claim 2 wherein said concave surface merges with said cover member in the vicinity of said transition portion.

4. The device of claim 1 wherein said juncture is located in the vicinity of the longitudinal midpoint of the tubular container portion.

5. The device of claim 1 wherein said slide portion base member constitutes a co-planar extension of the flat planar surface along the rear side of the container portion.

6. The device of claim 1 wherein said slide portion base member is parallel to but displaced slightly from the plane of said flat planar surface.

7. The device of claim 6 wherein said transition portion comprises a generally Z-shaped segment defining a wall joining the base member to the flat planar surface.

8. The device of claim 6 wherein said transition portion comprises a generally S-shaped wall having radiused terminal segments joining said base member and said flat planar surface.

9. The device of claim 1 wherein said chamber is bounded by closed side edges of said slide portion and by said closed terminal end and has an opening along a proximal end of the slide portion communicating with the exit opening of the tubular container portion.

10. The device of claim 9 wherein said chamber is approximately 0.009 to 0.0012 inch thick.

11. The device of claim 9 wherein said chamber further includes channel means extending from the chamber entrance opening along a major extent of the chamber to facilitate the movement of specimen particulates into said chamber and effect a more even distribution therein.

12. The device of claim 11 wherein said channel means comprise a pair of elongated channels extending longitudinally of the chamber along opposite edges thereof.

13. The device of claim 12 wherein said channels continue to a junction with each other across the terminal end of the chamber.

14. The device of claim 11 wherein said channel means comprise a generally V-shaped channel extending longitudinally of the chamber.

15. The device of claim 11 wherein the opening of the chamber and channel means is configured in a generally U-shaped transverse cross-section.

16. The device of claim 11 wherein the opening of the chamber and channel means is configured in a generally I-shaped transverse cross-section.

17. The device of claim 11 wherein the opening of the chamber and channel means is configured in a generally H-shaped transverse cross-section.

18. The device of claim 1 wherein the slide portion comprises a sandwich configuration including said chamber and said base and cover members wherein the base member is approximately 0.040 inch thick, the chamber is approximately 0.008 to 0.010 inch thick, and the cover member is approximately 0.009 to 0.012 inch thick, said sandwich configuration being approximately 0.060 inch thick.

19. A kit for the automatic preparation of a microscope slide in a centrifuge including the device of claim 1 in combination with a molded plastic cap for sealing said entrance opening, the configuration of the cap being shaped to mate with said entrance opening including the provision of a flat along one side to match the flat planar surface of the container portion.

20. The kit of claim 19 wherein said cap includes molded peripheral sealing rings extending about the periphery of the cap for sealingly engaging the inner wall of the container portion adjacent to the entrance opening.

21. The kit of claim 20 further including a funnel molded of flexible plastic, said funnel being adapted to be folded substantially flat for packing in the kit and restored to a generally circular configuration for engagement with the tubular container portion through said opening in order to facilitate the transfer of a specimen into said device.

22. The combination of claim 21 wherein said cap and said funnel are formed together as molded one-piece unit which is severable to remove the cap from the funnel.