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Qualitative immunochromatographic method and device

  • US 5,030,558 A
  • Filed: 11/07/1986
  • Issued: 07/09/1991
  • Est. Priority Date: 11/07/1986
  • Status: Expired due to Term
First Claim
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1. A method for determining the presence at or above a predetermined minimum detectible amount of one or more of a plurality of analytes in a sample suspected of containing one or more of said analytes, each analyte being a member of a specific binding pair ("sbp member") consisting of ligand and its complementary receptor, which method comprises(a) contacting, with a test solution containing said sample and predetermined amounts of two or more first sbp members each respectively analogous to one of said analytes, a contact portion of a piece of bibulous material capable of being traversed in at least one direction by said test solution by capillary migration, said bibulous material having substantially uniformly and non-diffusively bound thereto predetermined amounts of two or more second sbp members each respectively capable of binding one of said analytes and said first sbp members such that in the presence of a predetermined amount of each analyte the analogous first sbp member migrates at least to a predetermined site on said piece of bibulous material separated from said contact portion,(b) allowing at least a portion of the test solution to traverse said bibulous material by means of capillary migration at least to said predetermined site, and(c) detecting one or more of said first sbp members at said predetermined site.

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