Apparatus for detecting fluorescence of a luminescent material

• US 5,030,832 A
• Filed: 06/08/1989
• Issued: 07/09/1991
• Est. Priority Date: 06/08/1989
• Status: Expired due to Term

• Current Assignee: 3M Company
• Original Assignee: 3M Company
• Inventors: Williams, Richard E., Daniel, Mark L.
• Primary Examiner(s): Berman, Jack I.
• Assistant Examiner(s): Hanig, Richard
• Application Number:
  US07/364,194
• Time in Patent Office: 761 Days
• Field of Search: 250/458.1, 250/361 R, 250/361 C, 250/362, 250/459.1, 250/461.1, 250/461.2
• US Class Current: 250/458.1
• CPC Class Codes: G01N 2021/1748 Comparative step being essential in the method
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence

Abstract

An apparatus for detecting the fluorescence of a luminescent material. Photo-diodes receiving the visible fluorescence from a target luminescent material and a luminescent reference are coupled "back-to-back" resulting in a single-channel detector inherently balanced and insensitive to variations in the excitation stimulus. The photo-diode output is sent through a second harmonic amplifier making available a phase detection of the fluorescence instead of an amplitude detection. The phase of the signal is compared with a known phase from the excitation power source. By adding signals that either reinforce or oppose, the phase information is converted back to amplitude information at a voltage level that can be easily detected. The detected amplitude is compared against the amplitude of the excitation stimulus to account for amplitude variations in such stimulus. The compared output then triggers an indicator producing an indication of the amount of fluorescence of the target luminescent material.

Claims

1. An apparatus for detecting the fluorescence of a target luminescent material being excitable at an excitation wavelength and fluorescing at a fluorescing wavelength, comprising:

a reference luminescent material;

a discharge lamp adapted to be coupled to an alternating current voltage source operating at a source frequency and arranged to excite said target luminescent material and said reference luminescent material at approximately said excitation wavelength;

first response means arranged to be responsive to said target luminescent material at approximately said fluorescing wavelength for providing a first electrical response as a function of the electromagnetic energy received from said target luminescent material at said fluorescing wavelength;

second response means arranged to be responsive to said reference luminescent material for providing a second electrical response as a function of the electromagnetic energy received from said reference luminescent material;

said first response means and said second response means being arranged such that said first electrical response opposes said second electrical response creating a combined output signal;

a band pass filter coupled to said combined output signal selected to pass predominantly signals of a frequency of approximately twice said source frequency to produce a filtered output signal;

a phase detector coupled to said band pass filter to detect the phase of said filtered output signal and producing a first response when said filtered output signal is of a first phase and producing a second response when said filtered signal is of a second phase; and

an indicator coupled to said phase detector to indicate the condition of said fluorescence of said target luminescent material as a function of at least one of said first response and second response.

2. An apparatus for detecting fluorescence as in claim 1 wherein said first response means and said second response means comprise first and second photo-diodes operating in photo voltaic mode, said first and second photo-diodes being coupled back to back.

3. An apparatus for detecting fluorescence as in claim 2 wherein said phase detector comprises:

a rectifier adapted to be coupled to said alternating current voltage source producing a rectified output;

summing means coupled to said band pass filter and to said rectifier for summing said filtered output signal and said rectified output and producing a summed signal wherein a relative high magnitude for the amplitude of said summed signal is indicative of said first response and a relative low magnitude for the amplitude of said summed signal is indicative of said second response.

4. An apparatus for detecting fluorescence as in claim 3 wherein said phase detector further comprises an alternating current voltage source comparator coupled between said summing means and said indicator which compares the magnitude of the amplitude of said summed signal to the magnitude of the voltage amplitude of said alternating current voltage source and produces said first response as a function of the magnitude of amplitude of said line voltage.

5. An apparatus for detecting fluorescence as in claim 3 wherein said apparatus detects said fluorescence of said target luminescent material above a predetermined threshold and which further comprises an adjustment means coupled to said target luminescent material, to said reference luminescent material, to said first or second photo-diodes or to said discharge lamp for balancing the output of said first and second photo-diodes when said apparatus is measuring a target luminescent material which fluoresces at said predetermined threshold.

6. An apparatus for detecting fluorescence as in claim 4 which further comprises a phase delay coupled between said band pass amplifier and said phase detector to adjust the phase of said filtered output signal to maximize the opposition of the phase of said photo-diode output signal and said rectifier signal when said apparatus is measuring a target luminescent material which fluoresces at said predetermined threshold.

7. An apparatus for detecting the fluorescence of a target luminescent material being excitable at an excitation wavelength and fluorescing at a fluorescing wavelength comprising:

a reference luminescent material;

a discharge lamp adapted to be coupled to an alternating current voltage source operating at a source frequency and arranged to excite said target luminescent material and said reference luminescent material at approximately said excitation wavelength;

first response means arranged to be responsive to said target luminescent material at approximately said fluorescing wavelength for providing a first electrical response as a function of the electromagnetic energy received from said target luminescent material at said fluorescing wavelength;

second response means arranged to be responsive to said reference luminescent material for providing a second electrical response as a function of the electromagnetic energy received from said reference luminescent material;

said first response means and said second response means being arranged such that said first electrical response opposes said second electrical response creating a combined output signal;

phase conversion means coupled to said first and second photo-diodes for converting amplitude variations of said photo-diode output to phase variations producing a phase output signal;

a phase detector coupled to said phase output signal to detect the phase of said filtered output signal and producing a first response when said filtered output signal is of a first phase and producing a second response when said filtered signal is of a second phase; and

an indicator coupled to said phase detector to indicate the condition of said fluorescence of said target luminescent material as a function of at least one of said first response and second response.

8. An apparatus for detecting fluorescence as in claim 7 wherein said first response means and said second response means comprise first and second photo-diodes operating in photo voltaic mode, said first and second photo-diodes being coupled back to back.

9. An apparatus for detecting fluorescence as in claim 8 wherein said phase detector comprises:

a rectifier adapted to be coupled to said alternating current voltage source producing a rectified output;

summing means coupled to said phase converter and to said rectifier for summing said filtered output signal and said rectified output and producing a summed signal wherein a relative high magnitude for the amplitude of said summed signal is indicative of said first response and a relative low magnitude for the amplitude of said summed signal is indicative of said second response.

10. An apparatus for detecting fluorescence as in claim 9 wherein said phase detector further comprises an alternating current voltage source comparator coupled between said summing means and said indicator which compares the magnitude of the amplitude of said summed signal to the magnitude of the voltage amplitude of said alternating current voltage source and produces said first response as a function of the magnitude of amplitude of said line voltage.

11. An apparatus for detecting fluorescence as in claim 9 wherein said apparatus detects said fluorescence of said target luminescent material above a predetermined threshold and which further comprises an adjustment means coupled to said reference luminescent material, to said first or second photo-diode or to said discharge lamp for balancing output of said first and second photo-diodes when said apparatus is measuring a target luminescent material which fluoresces at said predetermined threshold.

12. An apparatus for detecting the fluorescence of a target luminescent material being excitable at an excitation wavelength and fluorescing at a fluorescing wavelength comprising:

a reference luminescent material;

a discharge lamp adapted to be coupled to an alternating current voltage source arranged to excite said target luminescent material and said reference luminescent material at approximately said excitation wavelength;

first response means arranged to be responsive to said target luminescent material at approximately said fluorescing wavelength for providing a first electrical response as a function of the electromagnetic energy received from said target luminescent material at said fluorescing wavelength;

second response means arranged to be responsive to said reference luminescent material for providing a second electrical response as a function of the electromagnetic energy received from said reference luminescent material;

said first response means and said second response means being arranged such that said first electrical response directly offsets said second electrical response creating a combined output signal;

an indicator coupled to said first response means and to second response means and responsive to said combined output signal to indicate the fluorescence of said target luminescent material based upon said combined output signal.

13. An apparatus for detecting fluorescence as in claim 12 wherein said first response means and said second response means comprise first and second photo-diodes operating in photo voltaic mode, said first and second photo-diodes being coupled back to back.

14. An apparatus for detecting fluorescence as in claim 13 wherein said apparatus detects said fluorescence of said target luminescent material above a predetermined threshold and which further comprises an adjustment means coupled to said reference luminescent material, to said first or second photo-diodes or to said discharge lamp for balancing the output of said first and second photo-diodes when said apparatus is measuring a target luminescent material which fluoresces at said predetermined threshold.

15. An apparatus for detecting the fluorescence of a target luminescent material being excitable at an excitation wavelength and fluorescing at a fluorescing wavelength comprising:

a discharge lamp adapted to be coupled to an alternating current voltage source operating at a source frequency and arranged to excite said target luminescent material at approximately said excitation wavelength;

a photo-diode being arranged to be responsive to said target luminescent material producing a photo-diode output signal;

a band pass filter coupled to said photo-diode output signal selected to pass predominantly signals of a frequency of approximately twice said source frequency to produce a filtered output signal;

an indicator coupled to said band pass filter and responsive to said filtered output signal to indicate the fluorescence of said target luminescent material based upon said filtered output signal.

16. An apparatus for detecting fluorescence as in claim 15 wherein said apparatus detects said fluorescence of said target luminescent material above a predetermined threshold and which further comprises an adjustment means coupled to said target luminescent material, to said photo-diode or to said discharge lamp for adjusting the output of said photo-diode when said apparatus is measuring a target luminescent material which fluoresces at said predetermined threshold.

17. An apparatus for detecting the fluorescence of a target luminescent material being excitable at an excitation wavelength and fluorescing at a fluorescing wavelength, comprising:

a discharge lamp adapted to be coupled to an alternating current voltage source operating at a source frequency and arranged to excite said target luminescent material at approximately said excitation wavelength;

a photo-diode being arranged to be responsive to said target luminescent material and operating in photo-voltaic mode and producing a photo-diode output signal;

a comparator coupled to said photo-diode which compares the magnitude of the amplitude of said photo-diode output signal to the magnitude of the voltage amplitude of said alternating current voltage source and produces a response as a function of the magnitude of amplitude of said voltage amplitude; and

an indicator coupled to said line voltage comparator and responsive to said photo-diode output signal to indicate the fluorescence of said target luminescent material based upon said photo-diode output signal.

18. An apparatus for detecting fluorescence as in claim 17 wherein said apparatus detects said fluorescence of said target luminescent material above a predetermined threshold and which further comprises an adjustment means coupled to said target luminescent material, to said photo-diode or to said discharge lamp for adjusting the output of said photo-diode when said apparatus is measuring a target luminescent material which fluoresces at said predetermined threshold.