Gene transfer using transformed, neodetermined, embryonic cells
First Claim
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1. A method of providing tissue-specific expression of exogenous genetic material is selected tissues of a recipient mammal which comprises:
- (a) providing carrier cells capable of selectively delivering said exogenous genetic material to said tissues, said cells selected from the group consisting of embryonic yolk sac cells, embryonic midbrain cells and embryonic forebrain cells, all of which are derived from a donor mammal of the same species, said cells also having been transformed in vitro with said exogenous genetic material and(b) introducing said cells into said recipient mammal in a manner permitting them to differentiate into said tissues.
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Abstract
This invention is directed to a method for the preparation of carrier cells capable of delivering exogenous genetic material to a particular tissue of the body by means of embryonic cells competent to develop into that tissue, and essentially only that tissue, said cells bearing the exogenous genetic material. The preferred carrier cells are mesodermal cells of the yolk sac or embryonic forebrain or midbrain cells, and the desired genetic material is preferably introduced into the cells by in vitro transformation with an amphotrophic retroviral vector.
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Citations
15 Claims
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1. A method of providing tissue-specific expression of exogenous genetic material is selected tissues of a recipient mammal which comprises:
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(a) providing carrier cells capable of selectively delivering said exogenous genetic material to said tissues, said cells selected from the group consisting of embryonic yolk sac cells, embryonic midbrain cells and embryonic forebrain cells, all of which are derived from a donor mammal of the same species, said cells also having been transformed in vitro with said exogenous genetic material and (b) introducing said cells into said recipient mammal in a manner permitting them to differentiate into said tissues. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification