Preparation of a selectively permeable membrane for an enzyme electrode
First Claim
1. An enzyme electrode comprising:
- an electrically conductive base,a selectively permeable membrane having an inner surface and an outer surface and inner surface being mounted to face the electrically conductive base and being prepared from a composition comprising a mixture of (1) an emulsion of resin and (2) a water-soluble polymer material, the emulsion of resin being curable by irradiation with ionizing radiation or ultraviolet ray and containing at least one prepolymer or monomer having ethylenic unsaturation, the selectively permeable membrane being obtained by curing the composition by irradiation with ionizing irradiation or ultraviolet ray, and wherein the selectively permeable membrane is permeable to a substance produced by an enzyme reaction while preventing the permeation of higher molecular weight materials, andan immobilized enzyme membrane formed on the outer surface of the selectively permeable membrane, wherein the enzyme is capable of producing the substance which can permeate the selectively permeable membrane which substance must pass through the selectively permeable membrane to contact the electrically conductive base.
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Accused Products
Abstract
A selectively permeable membrane for an enzyme electrode is prepared by forming a mixture of an emulsion of resin containing at least one prepolymer or monomer having ethylenic unsaturation, and a water-soluble polymer material, and subjecting the mixture to irradiation with ionizing or ultraviolet irradiation. The membrane is permeable to substances produced by an enzyme reaction while preventing the permeation of higher molecular weight materials. An immobilized enzyme membrane is formed on an outer surface of the permeable membrane, and the resultant membrane is mounted on an electrically conductive base to form an enzyme electrode. In another embodiment, the selectively permeable membrane is formed on the electrically conductive base, and then the immobilized enzyme membrane is formed. The selectively permeable membrane is situated between the immobilized enzyme membrane, which is in contact with a sample solution, and the electrically conductive base.
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Citations
19 Claims
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1. An enzyme electrode comprising:
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an electrically conductive base, a selectively permeable membrane having an inner surface and an outer surface and inner surface being mounted to face the electrically conductive base and being prepared from a composition comprising a mixture of (1) an emulsion of resin and (2) a water-soluble polymer material, the emulsion of resin being curable by irradiation with ionizing radiation or ultraviolet ray and containing at least one prepolymer or monomer having ethylenic unsaturation, the selectively permeable membrane being obtained by curing the composition by irradiation with ionizing irradiation or ultraviolet ray, and wherein the selectively permeable membrane is permeable to a substance produced by an enzyme reaction while preventing the permeation of higher molecular weight materials, and an immobilized enzyme membrane formed on the outer surface of the selectively permeable membrane, wherein the enzyme is capable of producing the substance which can permeate the selectively permeable membrane which substance must pass through the selectively permeable membrane to contact the electrically conductive base. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. An enzyme electrode comprising:
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an electrically conductive base, a selectively permeable membrane having an inner surface and an outer surface said inner surface being mounted on the electrically conductive base and said membrane being prepared from a composition comprising a mixture of (1) a first emulsion of resin and (2) a water-soluble polymer material, the first emulsion of resin being curable by irradiation with ionizing radiation or ultraviolet ray and containing at least one prepolymer or monomer having ethylenic unsaturation, the selectively permeable membrane being obtained by curing the composition by irradiation with ionizing irradiation or ultraviolet ray, and wherein the selectively permeable membrane is permeable to a substance produced by an enzyme reaction while preventing the permeation of higher molecular weight material, and an immobilized enzyme membrane formed on the outer surface of the selectively permeable membrane, the immobilized enzyme membrane being obtained by (a) applying on the outer surface of the selectively permeable membrane a composition containing a mixture of (1) a second emulsion of resin which is the same as or different than the above said first emulsion resin and is curable by irradiation of ionizing radiation or ultraviolet ray, and (2) an enzyme which is capable of producing the above said substance which can permeate the selectively permeable membrane, and (b) curing the composition by irradiation with ionizing radiation or ultraviolet ray. - View Dependent Claims (12, 13, 14)
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15. An enzyme electrode comprising:
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an electrically conductive base, a selectively permeable membrane having an inner surface and an outer surface said inner surface being mounted on the electrically conductive base, said membrane being prepared from a composition comprising a mixture of (1) a first emulsion of resin and (2) a first water-soluble polymer material, the first emulsion of resin being curable by irradiation with ionizing radiation or ultraviolet ray and containing at least one prepolymer or monomer having ethylenic unsaturation, the selectively permeable membrane being obtained by curing the composition by irradiation with ionizing irradiation or ultraviolet ray, and the selectively permeable membrane being permeable to a substance produced by an enzyme reaction while preventing the permeation of higher molecular weight materials, and an immobilized enzyme membrane formed on the outer surface of the selectively permeable membrane, the immobilized enzyme membrane being obtained by (a) applying on the outer surface of the selectively permeable membrane a composition containing a mixture of (1) a second emulsion of resin which is the same as or different than the above said first emulsion of resin and which is curable by irradiation of ionizing radiation or ultraviolet ray, (2) a second water-soluble polymer material which is the same as or different than the above said first water-soluble polymer material, and (3) an enzyme which is capable of producing the above said substance which can permeate the selectively permeable membrane, and (b) curing the composition by irradiation with ionizing radiation or ultraviolet ray. - View Dependent Claims (16, 17)
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18. A process of manufacturing an enzyme electrode comprising the steps of:
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(a) forming a composition comprising a mixture of (1) an emulsion of resin containing at least one prepolymer or monomer having ethylenic unsaturation curable by irradiation with ionizing radiation or ultraviolet ray and (2) a water-soluble polymer material; (b) irradiating the composition with ionizing radiation or ultraviolet ray to cure the composition and thereby form a selectively permeable membrane having an inner surface and an outer surface and which is permeable to a substance produced by an enzyme reaction while preventing the permeation of higher molecular weight substances; (c) forming an immobilized enzyme membrane on the outer surface of the selectively permeable membrane; and (d) mounting the selectively permeable membrane having the immobilized enzyme membrane formed thereon with an electrically conductive base so that the inner surface of the selectively permeable membrane faces the said base.
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19. A process of manufacturing an enzyme electrode comprising the steps of:
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(a) forming a composition comprising a mixture of (1) an emulsion of resin containing at least one prepolymer or monomer having ethylenic unsaturation curable by irradiation with ionizing radiation or ultraviolet ray and (2) a water-soluble polymer material, on an electrically conductive base; (b) irradiating the composition with ionizing radiation or ultraviolet ray to cure the composition and thereby form a selectively permeable membrane having an inner surface and an outer surface and which is permeable to a substance produced by an enzyme reaction while preventing the permeation of higher molecular weight substances the said inner surface being adjacent to said electrically conductive base; and (c) forming an immobilized enzyme membrane on the outer surface of the selectively permeable membrane.
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Specification