Method for transporting substances into living cells and tissues and apparatus therefor

• US 5,036,006 A
• Filed: 08/17/1989
• Issued: 07/30/1991
• Est. Priority Date: 11/13/1984
• Status: Expired due to Term

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Sanford, John C., Wolf, Edward D., Allen, Nelson K.
• Primary Examiner(s): Lacey, David L.
• Assistant Examiner(s): Beisner, William H.
• Application Number:
  US07/394,899
• Time in Patent Office: 712 Days
• Field of Search: 435/170.1, 435/29, 435/34, 435/35, 435/172.3, 435/173, 435/240, 435/240.2, 435/240.4, 435/243, 435/252.3, 435/254.6, 435/7, 435/235, 935/52, 935/53, 424/3, 424/7.1, 222/630, 604/131, 604/140, 604/141, 604/143, 604/68, 604/70
• US Class Current: 435/459
• CPC Class Codes: C12M 35/00 Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion machines for extracting juice from animal or plant tissue by electroplasmolysis A23N1/006, processes employing electric or wave energy B01J19/08; treatment of microorganisms or enzymes with electrical or wave energy C12N13/00; methods for cell fusion C12N15/02; introduction of foreign genetic material C12N15/87
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: C12N 15/8207 by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
• CPC Class Codes: C12N 15/87 Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

Inert or biologically active particles are accelerated or propelled at cells at a speed such that the particles can penetrate the surface of the cells and become incorporated into the interior of the cells. The process can be used to mark cells or tissue or to biochemically affect tissues or tissue in situ as well as single cells in vitro. Apparatus for accelerating or propelling the particles toward target cells or tissues are also disclosed. A method for releasing particles adhered to a rotor device is also disclosed.

Claims

1. A method for introducing particles into cells comprising accelerating particles having a diameter sufficiently small to penetrate and be retained in a preselected cell without killing the cell, and propelling said particles at said cells whereby said particles penetrate the surface of said cells and become incorporated into the interior of said cells wherein the cells are cells of monocot plants.

2. A method as in claim 1, wherein said cells of monocot plants are cells of graminaceous plants.

3. A method as in claim 2, wherein said cells of graminaceous plants are cells of a plant selected from the group consisting of wheat, rice and corn.

4. A method for introducing particles into cells comprising accelerating particles having a diameter sufficiently small to penetrate and be retained in a preselected cell without killing the cell, and propelling said particles at said cells whereby said particles penetrate the surface of said cells and become incorporated into the interior of said cells wherein the cells are cells of dicot plants.

5. A method as in claim 4, wherein said cells of dicot plants are cells of a plant selected from the group consisting of tobacco and apple.

6. A method for introducing particles into cells comprising accelerating particles having a diameter sufficiently small to penetrate and be retained in a preselected cell without killing the cell, and propelling said particles at said cells whereby said particles penetrate the surface of said cells and become incorporated into the interior of said cells wherein the cells are animal cells or fungal cells.

7. A method for introducing particles into cells comprising accelerating particles having a diameter sufficiently small to penetrate and be retained in a preselected cell without killing the cell, and propelling said particles at said cells whereby said particles penetrate the surface of said cells and become incorporated into the interior of said cells wherein the particles comprise inert particles associated with viral nucleic acids in a biologically active form capable of replication inside the cell.

8. A method of introducing particles into cells comprising accelerating particles having a diameter sufficiently small to penetrate and be retained in a preselected cell without killing the cell, and propelling said particles at said cells whereby said particles penetrate the surface of said cells and become incorporated into the interior of said cells wherein the particles comprise inert particles associated with RNA in a biologically active form capable of replication or translation inside the cell.

9. A method for introducing particles into cells comprising accelerating particles having a diameter sufficiently small to penetrate and be retained in a preselected cell without killing the cell, and propelling said particles at said cells whereby said particles penetrate the surface of said cells and become incorporated into the interior of said cells wherein the particles comprise inert particles associated with DNA and the cells are capable of subsequently expressing genes encoded by said DNA.

10. A method as in claim 9 where the cells are plant cells.

11. A method as in claim 10, wherein said plant cells are cells of monocot plants.

12. A method for introducing particles into cells comprising accelerating particles having a diameter sufficiently small to penetrate and be retained in a preselected cell without killing the cell, and propelling said particles at said cells whereby said particles penetrate the surface of said cells and become incorporated into the interior of said cells, wherein said accelerating is by magnetic means.

13. A method for introducing particles into cells comprising accelerating particles having a diameter sufficiently small to penetrate and be retained in a preselected cell without killing the cell, and propelling said particles at said cells whereby said particles penetrate the surface of said cells and become incorporated into the interior of said cells, wherein said accelerating is by an expanding gas means.