Simultaneous assay for glucose and urea
First Claim
1. A method for the simultaneous determination of glucose and urea in a sample with a single reagent system in a reaction mixture comprising:
- adding in a single step a reagent system containing enzymatic reagents and reactants for the simultaneous determination of glucose and urea, each enzymatic reagent and reactant being selected such that it capable of contributing to a discrete electromagnetic radiation signal for either glucose or urea, wherein the determination of glucose does not interfere with the determination of urea, and the determination of urea does not interfere with the determination of glucose;
simultaneously reacting a sample containing glucose and urea with the reagent system; and
monitoring changes in absorbance or fluorescence of the resulting reaction mixture at two or more wavelengths, thereby separately but simultaneously determining the concentration of glucose and urea in the sample.
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Abstract
A method for the simultaneous determination of glucose and urea in a specimen with a single reagent system. A reagent system containing a reactant for each of glucose and urea to be determined is added to a specimen. The reagent system is reacted with the specimen such that each of glucose and urea react with their respective reactant simultaneously. Each reactant is selected such that it is capable of giving an absorbance band for glucose and urea which permits their simultaneous determination. The change in absorbance or fluorescence of the resulting reaction mixture is monitored at a plurality of wavelengths which are characteristic for each of glucose and urea.
23 Citations
34 Claims
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1. A method for the simultaneous determination of glucose and urea in a sample with a single reagent system in a reaction mixture comprising:
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adding in a single step a reagent system containing enzymatic reagents and reactants for the simultaneous determination of glucose and urea, each enzymatic reagent and reactant being selected such that it capable of contributing to a discrete electromagnetic radiation signal for either glucose or urea, wherein the determination of glucose does not interfere with the determination of urea, and the determination of urea does not interfere with the determination of glucose; simultaneously reacting a sample containing glucose and urea with the reagent system; and monitoring changes in absorbance or fluorescence of the resulting reaction mixture at two or more wavelengths, thereby separately but simultaneously determining the concentration of glucose and urea in the sample. - View Dependent Claims (30)
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2. A method for the simultaneous determination of glucose and urea in a sample with a single reagent system in a reaction mixture comprising:
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adding in a single step a reagent system containing enzymatic reagents and chromophores for the simultaneous determination of glucose and urea, each enzymatic reagent and chromophore being selected such that it is capable of contributing to a discrete electromagnetic radiation absorbance signal for either glucose or urea, wherein the determination of glucose does not interfere with determination of urea, and the determination of urea does not interfere with the determination of glucose; simultaneously reacting a sample containing glucose and urea with the reactant system; and monitoring changes in absorbance or fluorescence of the resulting reaction mixture at two or more wavelengths, thereby separately but simultaneously determining the concentration of glucose and urea, in the sample. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 31, 32, 33, 34)
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Specification