Sensor element for determination of concentration of substances

• US 5,039,490 A
• Filed: 04/17/1987
• Issued: 08/13/1991
• Est. Priority Date: 04/23/1986
• Status: Expired due to Fees

• Current Assignee: AVL AG
• Original Assignee: AVL AG
• Inventors: Kroneis, Herbert, Karpf, Hellfried, Wolfbeis, Otto S., List, Helmut, Leitner, Alfred, Marsoner, Hermann
• Primary Examiner(s): Simmons, David A.
• Assistant Examiner(s): DANG, THI D
• Application Number:
  US07/040,163
• Time in Patent Office: 1,579 Days
• Field of Search: 436/172, 436/164, 436/805, 436/806, 422/55, 422/57, 422/82.01, 422/82.09, 422/88, 422/90, 422/91, 356/435, 356/219, 356/222, 356/402, 356/416, 356/445, 356/446, 250/216, 250/574
• US Class Current: 422/82.01
• CPC Class Codes: G01N 2021/174 either absorption-reflection or emission-fluorescence
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 21/81 Indicating humidity
• CPC Class Codes: G01N 2201/0621 Supply
• CPC Class Codes: G01N 2201/0622 Use of a compensation LED

Abstract

Due to the size of the devices used for the excitation and measuring of light, conventional sensor elements for optically determining the concentrations of substances contained in gaseous and liquid samples, featuring an indicator layer with one or more indicator substances, are not well suited for use with microanalysis equipment, nor are they easy to mass-produce. These disadvantages are eliminated by using a unitary sensor element having a carrier layer, at least one photosensitive element with electric contact leads in contact with the carrier layer, and an indicator layer containing an indicator substance.

Claims

1. An electro-optical sensor element for determining the concentration of substances contained in gaseous and liquid samples, comprising

a carrier layer,

a thin-film substrate comprising at least one photosensitive element, a plurality of electrical contacts and at least one area which is transparent to excitation radiation associated with said carrier layer by microelectronic and photolithographic techniques,

an indicator layer, and

a transparent coupling layer located between said thin-film substrate and said indicator layer, said transparent coupling layer containing a diffraction structure,

said indicator layer being produced by microelectronic and photolithographic techniques and comprising at least one indicator substance having optical properties, at least one of the optical properties of said indicator substance changing upon interaction with said substances contained in said gaseous and liquid samples depending on the concentrations of said substances in said gaseous and liquid samples, and wherein said indicator substance of said indicator layer, which is excited by a source of excitation radiation, is in optical contact with said at least one photosensitive element via said transparent coupling layer, said diffraction structure directing said excitation radiation into areas of said indicator layer facing said at least one photosensitive element.

2. An electro-optical sensor element according to claim 1, wherein said diffraction structure is a periodic lattice structure.

6. An electro-optical sensor element according to claim 1, 3 or 4, wherein one end of a lightguide extends to said areas transparent to said excitation radiation.

7. An electro-optical sensor element according to claim 1, 3 or 4, wherein said areas transparent to said excitation radiation are provided with a filter layer.

8. An electro-optical sensor element according to claim 7, wherein said filter layer is an optical interference filter.

9. An electro-optical sensor element according to claim 1, 3 or 4, wherein said photosensitive elements are additionally coated with an optical filter layer.

10. An electro-optical sensor element according to claim 1, 3 or 4, wherein an interference filter is situated between said coupling layer and said indicator layer, which has different transmission coefficients for said excitation radiation and said fluorescent radiation emitted by said indicator substance.

11. An electro-optical sensor element according to claim 1, 3 or 4, wherein said indicator layer is provided with microareas corresponding to individual photosensitive elements and containing different indicators.

12. An electro-optical sensor element according to claim 1, 3 or 4, wherein said indicator layer is made of porous glass containing said indicator in an immobilized form.

13. An electro-optical sensor element according to claim 1, 3 or 4, wherein said indicator layer is a silicone film which is applied by spinning or rolling, and in which said indicator is contained.

14. An electro-optical sensor element according to claim 1, 3 or 4, wherein indicator molecules of said indicator substance are chemically bonded to said transparent coupling layer.

15. An electro-optical sensor element according to claim 1, 3 or 4, wherein said indicator substances are deposited by techniques such as micro-screenprinting or evaporation coating.

16. An electro-optical sensor element according to claim 1, 3 or 4, wherein electronic circuits are integrated on said carrier layer, said circuits being designed for amplifying electric signals produced by said photosensitive elements.

17. An electro-optical sensor element according to claim 16, wherein said carrier layer is provided with highly integrated electronic circuits for signal evaluation tasks.

18. An electro-optical sensor element according to claim 1, 3 or 4, wherein said microelectronic and photolithographic techniques comprise evaporation coating, sputtering, spinning, etching and ion beam erosion.

3. An electro-optical sensor element for determining the concentration of substances contained in gaseous and liquid samples, comprising

a carrier layer,

a thin film substrate comprising at least one photosensitive element, a plurality of electrical contacts and at least one area which is transparent to excitation radiation associated with said carrier layer by microelectronic and photolithographic techniques,

an indicator layer,

a transparent coupling layer located between said thin-film substrate and said indicator layer,

said indicator layer being produced by microelectronic and photolithographic techniques and comprising at least one indicator substance having optical properties, wherein at least one of the optical properties of said indicator substance will change upon interaction with said substances contained in said gaseous and liquid samples depending on the concentrations of said substances in said gaseous and liquid samples, and wherein said indicator substance of said indicator layer, which is excited by a source of excitation radiation, is in optical contact with said at least one photosensitive element via said transparent coupling layer, and

transparent areas positioned in said thin-film substrate at an angle, such that said excitation radiation will strike said indicator layer at an angle a of 40°

to 60°

, exciting areas of said indicator layer facing said photosensitive elements.

4. An electro-optical sensor element for determining the concentration of substances contained in gaseous and liquid samples, comprising

a carrier layer,

a thin film substrate comprising at least one photosensitive element, a plurality of electrical contacts and at least one area which is transparent to excitation radiation associated with said carrier layer by microelectronic and photolithographic techniques,

an indicator layer,

a transparent coupling layer located between said thin-film substrate and said indicator layer,

said indicator layer being produced by microelectronic and photolithographic techniques and comprising at least one indicator substance having optical properties, wherein at least one of the optical properties of said indicator substance will change upon interaction with said substances contained in said gaseous and liquid samples depending on the concentrations of said substances in said gaseous and liquid samples, and wherein said indicator substance of said indicator layer, which is excited by a source of excitation radiation, is in optical contact with said at least one photosensitive element via said transparent coupling layer, and wherein said coupling layer, which has a refractive index n₂, together with boundary layers situated on both sides of said coupling layer, which have a refractive index n₁, constitute a planar lightguide for said radiation to be measured, with n₂ >

n₁, wherein said boundary layer adjacent to said sample has at least one recess which is covered by said indicator layer, and wherein each of said recesses, seen in a direction of said excitation radiation, is situated above one of said areas transparent to said excitation radiation, and wherein total reflection of said radiation to be measured is cancelled in areas of said photosensitive elements.

5. An electro-optical sensor element according to claim 4, with only one of said areas transparent to said excitation radiation and wherein said photosensitive element has an annular structure concentric to said area.