Osmotic agents for peritoneal dialysis

• US 5,039,609 A
• Filed: 04/18/1988
• Issued: 08/13/1991
• Est. Priority Date: 09/10/1985
• Status: Expired due to Fees

• Current Assignee: Research Corporation Technologies Incorporated 6840 East Broadway Boulevard Tucson
• Original Assignee: Research Corporation Technologies
• Inventors: Klein, Elias
• Primary Examiner(s): Stone, Jacqueline
• Application Number:
  US07/182,708
• Time in Patent Office: 1,212 Days
• Field of Search: 514/2, 514/21, 530/407, 530/833, 530/414, 435/68.1, 435/72, 435/272, 435/275, 210/632, 210/652, 210/96.2, 210/196
• US Class Current: 435/68.1
• CPC Class Codes: A61K 38/018 from milk
• CPC Class Codes: A61M 1/287 Dialysates therefor
• CPC Class Codes: A61P 3/00 Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: Y10S 530/833 Whey; cheese

Abstract

This invention relates to a peritoneal dialysis solution which comprises as an osmotically active agent an osmotically effective amount of a mixture of peptides, the mixture consisting substantially of peptides having a molecular weight of about 300 to about 2000 daltons, and an equivalent weight between about 150 to about 1500.

Claims

1. A process for preparing low molecular weight peptides having a molecular weight between about 300 to about 2000 daltons which comprises:

a) treating a high quality protein in an aqueous solution under hydrolyzing conditions, with at least one hydrolytic enzyme, to produce a peptide-containing solution, at least some of the peptides having a low molecular weight between about 300 to about 2000 daltons and at least some of the peptides having a molecular weight above about 2000 daltons;

b) passing the peptide-containing solution into contact with one side of a dialysis membrane while substantially pure water, obtained from step (d) herein is passing in contact with the other side of said dialysis membrane under effective dialysis separation conditions, said dialysis membrane being effective to allow transport therethrough of the low molecular weight peptides having molecular weight between about 300 to about 2000 daltons while retaining the peptides having a molecular weight above about 2000 daltons in the peptide-containing solution and said water having a sufficiently low peptide solute concentration effective to allow transport of the low molecular weight peptides across the membrane into the water;

c) passing the water and the transported low molecular weight peptides contained therein from the other side of the dialysis membrane to a reservoir;

d) passing water, containing transported low molecular weight peptides, from the reservoir into contact with one side of a reverse osmosis (RO) membrane under reverse osmosis separation conditions, including pressure, effective to cause substantially pure water to be transported across said RO membrane to provide a quantity of substantially pure water for use in step (b) herein and to retain at said one side of said RO membrane a quantity of water containing the transported low molecular weight peptides at a higher concentration than in the water passing to the RO membrane; and

e) passing the water containing the transported low molecular weight peptides at a higher concentration from said one side of said RO membrane to the reservoir to provide in the reservoir an aqueous solution of low molecular weight peptides at a concentration substantially higher than in the water containing the transported low molecular weight peptides passing to the reservoir in step (c), herein.

2. The process of claim 1 wherein the aqueous solution containing retained peptides having a molecular weight of above about 2000 daltons are recycled from step (b) to step (a).

3. The process of claim 2 wherein the process is repeated until the concentration of the solution in the reservoir is sufficient to prevent production of pure water from the reverse osmosis unit.

4. The process of claim 2 wherein the process is repeated until the high quality protein is substantially depleted.

5. The process of claim 1 wherein hydrolysis is conducted in an enzyme reactor.

6. The process of claim 1 wherein the pressure on the reverse osmosis unit is between about 200-300 psi.

7. The process of claim 1 wherein the dialysis membrane is composed of cellulose.

8. The process of claim 7 wherein the membrane is a hollow fiber membrane.

9. The produce produced according to the process of claim 1.