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Microtuber propagation of potatoes

  • US 5,047,343 A
  • Filed: 04/29/1988
  • Issued: 09/10/1991
  • Est. Priority Date: 04/29/1988
  • Status: Expired due to Fees
First Claim
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1. A method of inducing tuber formation at a rate of more than two microtubers from a single potato microtuber explant comprising:

  • (a) culturing a potato microtuber in vitro so that a shoot is formed therefrom;

    (b) inducing a microtuber-shoot complex formation containing multiple independently-growing shoot axes by culturing the microtuber in a medium sufficiently low in calcium so as to induce shoot-tip necrosis on the shoot growing from the microtuber;

    (c) placing the microtuber-shoot complex in a growth medium under conditions suitable for increasing the length of the shoot axes and creating a root structure for the shoot axes; and

    (d) placing the microtuber shoot complex under conditions for encouraging the tuberization of the shoots so that multiple microtubers are formed on the independently-growing shoots.

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