Microtuber propagation of potatoes
First Claim
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1. A method of inducing tuber formation at a rate of more than two microtubers from a single potato microtuber explant comprising:
- (a) culturing a potato microtuber in vitro so that a shoot is formed therefrom;
(b) inducing a microtuber-shoot complex formation containing multiple independently-growing shoot axes by culturing the microtuber in a medium sufficiently low in calcium so as to induce shoot-tip necrosis on the shoot growing from the microtuber;
(c) placing the microtuber-shoot complex in a growth medium under conditions suitable for increasing the length of the shoot axes and creating a root structure for the shoot axes; and
(d) placing the microtuber shoot complex under conditions for encouraging the tuberization of the shoots so that multiple microtubers are formed on the independently-growing shoots.
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Abstract
The production of potatoes by growing a large number of independent shoot axes, each of which will form one or more microtubers, from a single microtuber is disclosed. This system has three interconnected stages: (1) the formation of a microtuber shoot complex by inducing shoot tip necrosis in the apical shoot; (2) the elongation of the resulting multiple shoot axes, and (3) the tuberization of the multiple shoot axes.
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Citations
19 Claims
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1. A method of inducing tuber formation at a rate of more than two microtubers from a single potato microtuber explant comprising:
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(a) culturing a potato microtuber in vitro so that a shoot is formed therefrom; (b) inducing a microtuber-shoot complex formation containing multiple independently-growing shoot axes by culturing the microtuber in a medium sufficiently low in calcium so as to induce shoot-tip necrosis on the shoot growing from the microtuber; (c) placing the microtuber-shoot complex in a growth medium under conditions suitable for increasing the length of the shoot axes and creating a root structure for the shoot axes; and (d) placing the microtuber shoot complex under conditions for encouraging the tuberization of the shoots so that multiple microtubers are formed on the independently-growing shoots. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A potato microtuber-shoot complex in vitro comprising;
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a seed microtuber; a plurality of axillary shoots originating from and connected to the seed microtuber, the axillary shoots having root formation; and a daughter microtuber formed on at least two of the axillary shoots.
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Specification