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Optical end-point type analytical method

  • US 5,047,351 A
  • Filed: 05/10/1989
  • Issued: 09/10/1991
  • Est. Priority Date: 09/01/1986
  • Status: Expired due to Term
First Claim
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1. A method of analyzing the concentration of an analyte using the end-point method and plural predetermined calibration curves showing the variation of the optical density or emission intensity with analyte concentrations at various predetermined time intervals by observing the variation in optical density or emission intensity of a sample occurring due to the reaction of the analyte, which comprises:

  • (a) measuring the optical density or emission intensity due to the reaction of the analyte at least once at a predetermined time interval after the beginning of the reaction;

    (b) if the optical density or emission intensity obtained at the first measurement is smaller than a predetermined critical value (TH1), selecting a predetermined calibration curve showing the variation of the optical density or emission intensity with analyte concentrations at various predetermined time intervals for a reaction time corresponding to said first measurement, and discontinuing the measurement,(c) if the optical density or emission intensity obtained at the first measurement is not smaller than a predetermined critical value (TH1), continuing the reaction further until an optical density or emission intensity smaller than TH1 wherein 1 is at least 2 and TH1 is greater than TH1-1 for each time interval T1 is obtained, then selecting a predetermined calibration curve showing the variation of optical density or emission intensity with analyte concentration at the predetermined time interval for a reaction time corresponding to the measurement where the optical density or emission intensity smaller than TH1 is obtained for the first time, and discontinuing the measurement, and(d) determining the amount of the analyte using said selected calibration curve.

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