Configuration fiber-optic blood gas sensor bundle and method of making

• US 5,047,627 A
• Filed: 05/18/1990
• Issued: 09/10/1991
• Est. Priority Date: 05/18/1990
• Status: Expired due to Term

• Current Assignee: Hospira Incorporated (Pfizer Inc.)
• Original Assignee: Abbott Laboratories
• Inventors: Hubbard, Todd W., Melkerson, Lori D., Sexton, Michael A., Fieggen, Bruce M., Yim, Jeffrey B.
• Primary Examiner(s): Nelms, David C.
• Assistant Examiner(s): Messinger, Michael
• Application Number:
  US07/526,185
• Time in Patent Office: 480 Days
• Field of Search: 356/41, 356/40, 356/39, 128/634, 128/637, 250/227.23, 250/226
• US Class Current: 250/227.23
• CPC Class Codes: G01N 2021/3181 using LEDs
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 21/783 for analysing gases
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

A bundle of fiber-optic sensors that resist breakage, for use in measuring pH, CO₂, and O₂ concentration, and a method of making the same. Three otpical fibers are enclosed in a common polyimide sheath and extend parallel to one another toward a distal end of the sensor, where they are bonded together in a mutually supportive array. At the distal end of one of these optical fibers is disposed a pellet comprising a CO₂ analyte indicator molecule codissolved within a polymer matrix. A thin reflective surface of gold foil is provided on the pellet such that incident light transmitted through the optical fiber and polymer matrix is reflected back into the optical fiber. The CO₂ polymer matrix absorbs light of a given wavelength to an extent dependent upon the level of CO₂ present. Similarly, on the distal end of a second optical fiber is disposed a pH pellet comprising a pH analyte indicator matrix with a thin reflective gold foil attached for reflecting light that has passed through the pH analyte indicator matrix back into the optical fiber. The pH analyte indicator matrix absorbs light of a given wavelenth to an extent dependent upon the hydrogen ion concentration (pH level) of a surrounding fluid. The third optical fiber is used for measuring oxygen concentration. An O₂ indicator matrix covers the distal ends of at least two of the three optical fibers; however, at least a portion of the pH optical fiber and the pH pellet are free of the O₂ indicator matrix, which is hydrophobic. The oxygen indicator matrix phosphoresces for an interval of time that decreases in proportion to the surrounding oxygen gas concentration. The phosphorescent and reflected light signals are transmitted to light detectors through the optical fibers for comparison to reference signals, so that the analyte concentrations can be determined.

Claims

1. A multi-analyte sensor comprising:

(a) a first, a second, and a third optical fiber, each optical fiber having a longitudinal axis and a distal end, the longitudinal axis corresponding to the direction that a light signal propagates through said optical fibers, all of said optical fibers being arranged in a sheath such that their longitudinal axes are substantially parallel, said sheath terminating short of the distal ends of the optical fibers, leaving an exposed portion of each optical fiber extending beyond the sheath, only two of the optical fibers being bonded together up to and around their distal ends, the distal ends of the optical fibers forming a mutually supporting array;

(b) a first indicator matrix containing a first indicator molecule, disposed adjacent to said first optical fiber, said first indicator molecule exhibiting a first signal in response to light at a first wavelength to an extent that depends upon a first analyte concentration around the sensor;

(c) a second indicator matrix containing a second indicator molecule, disposed adjacent to said second optical fiber, said second indicator molecule exhibiting a second signal in response to light at a second wavelength to an extent that depends upon a second analyte concentration around the sensor; and

(d) a third indicator matrix containing a third indicator molecule, disposed adjacent said third optical fiber, said third indicator molecule exhibiting a third signal in response to light of a third wavelength to an extent that depends upon a third analyte concentration around the sensor, wherein said mutually supporting array minimizes breakage of any of the optical fibers and indicator matrices due to handling.

2. The multi-analyte sensor of claim 1, further comprising a first light reflectance material disposed adjacent said first indicator matrix and a second light reflectance material disposed adjacent said second indicator matrix, wherein said first and second signals are reflected from said first and second light reflectance materials and are indicative of the absorbance of light at said first and said second wavelengths as a function of the first and second analyte concentrations, respectively, and wherein said third signal is the phosphorescence of said third indicator molecule in response to light at said third wavelength, which varies as a function of the third analyte concentration.

3. The multi-analyte sensor of claim 1, wherein said optical fibers are aligned in a triangular array adjacent their distal ends.

4. The multi-analyte sensor of claim 1, wherein at least one of the first, second, and third indicator matrices absorbs light as a function of one of the first, second, and third analyte concentrations, and at least another of said indicator matrices emits light when excited, said light emission continuing for a time interval that is a function of another of said analyte concentrations.

5. The multi-analyte sensor of claim 1, wherein at least one indicator matrix is substantially pellet shaped and is attached to a substantially planar face of the distal end of a corresponding one of the optical fibers.

6. The multi-analyte sensor of claim 1, wherein the distal ends of said first, second, and third optical fibers are overcoated such that said multi-analyte sensor has a hydrodynamic shape.

7. The multi-analyte sensor of claim 1, wherein two of said optical fibers each extend distally beyond the other of the optical fibers.

8. The multi-analyte sensor of claim 1, wherein said first indicator matrix and said second indicator matrix are substantially pellet shaped, and are attached to a planar face at the distal end of said first optical fiber and said second optical fiber, respectively.

9. The multi-analyte sensor of claim 1, wherein the distal ends of at least two of said optical fibers are overcoated with a hydrophobic material.

10. The multi-analyte sensor of claim 1, wherein the distal end of at lest one of the optical fibers is overcoated with a hydrophilic material.

11. The multi-analyte sensor of claim 1, wherein said first indicator matrix and said second indicator matrix each comprise substantially cylindrically shaped pellets, and said pellets are attached to transverse planar surfaces formed on the distal end of said first optical fiber and said second optical fiber, respectively, each pellet including a light reflective layer disposed opposite the transverse planar surface of the optical fiber to which the pellet is attached, each said light reflective layers on the pellets reflecting light exiting the transverse planar surface of the respective first and second optical fibers back into said optical fibers.

12. The multi-analyte sensor of claim 11, wherein the distal ends of said first, second, and third optical fibers are overcoated such that said multi-analyte sensor has a hydrodynamic shape.

13. The multi-analyte sensor of claim 11, wherein the distal end of one of the optical fibers is not overcoated and the distal ends of the other two optical fibers are overcoated with at least one of the indicator matrices in order to bond the two optical fibers together.

14. A medical sensor for a plurality of analytes comprising:

a plurality of optical fibers having distal ends extending longitudinally in parallel alignment, said optical fibers being enclosed in a common protective sheath over at least part of their length, but exposed at their distal ends, only two of said optical fibers being configured and bonded together by a matrix material that encompasses their distal ends;

a plurality of different analyte indicator materials, each analyte indicator material sensitive to the concentration of one of the plurality of analytes, each analyte indicator material being disposed generally at the distal ends of the optical fibers so as to vary a light signal propagated through the optical fibers as a function of the concentration of a different one of the analytes, at least one of the analyte indicators comprising the matrix material used to bond the two optical fibers together;

wherein the optical fibers form a mutually supportive array that resists breakage of any one of the optical fibers comprising the array.

15. A method for manufacturing a blood gas sensor comprising the steps of:

(a) aligning a first, a second, and a third optical fiber within a sheath such that the longitudinal axes of all said optical fibers are substantially parallel, all of the optical fibers having distal ends that extend beyond where the sheath terminates so that the distal ends are exposed;

(b) applying a first indicator matrix containing a first indicator molecule adjacent to the distal end of said first optical fiber, said first indicator molecule exhibiting a first signal in response to light at a first wavelength to an extent dependent upon a first analyte concentration around the sensor;

(c) applying a second indicator matrix containing a second indicator molecule adjacent to the distal end of said second optical fiber, said second indicator molecule exhibiting a second signal in response to light at a second wavelength to an extent dependent upon a second analyte concentration around the sensor; and

(d) applying a third indicator matrix material containing a third indicator molecule up to and around said distal ends of only two of said optical fibers thereby bonding the exposed portion of the two optical fibers together, said third indicator molecule exhibiting a third signal in response to light of a third wavelength to an extent which depends upon a third analyte concentration around the sensor, the two optical fibers bonded together providing a mutually supportive array, so that all of said optical fibers mutually reinforce each other and are resistant to breakage.

16. The method of claim 15, wherein the step of aligning all of said optical fibers comprises the step of aligning said optical fibers in a triangular array.

17. The method of claim 15, wherein the step of aligning comprises the step of positioning the distal ends of the optical fibers so that they are not all transversely aligned.

18. The method of claim 16, wherein prior to the alignment of said optical fibers, all of said optical fibers are overcoated with a thin coat of a protective polymer.

19. The method of claim 16, further comprising the step of overcoating at least two of said optical fibers with a hydrophobic material.

20. The method of claim 16, wherein the step of applying one of said first and said second indicator matrices comprises the step of affixing a pellet to one of said first and said second optical fibers.

21. The method of claim 20, wherein said pellet comprises a light reflectance material.

22. The method as claimed in claim 16, further comprising the step of overcoating the distal ends of the optical fibers to form a hydrodynamic shape.