Breeding apparatus

• US 5,049,505 A
• Filed: 05/17/1990
• Issued: 09/17/1991
• Est. Priority Date: 09/27/1988
• Status: Expired due to Fees

• Current Assignee: Kabushiki Kaisha Komatsu Seisakusho
• Original Assignee: Kabushiki Kaisha Komatsu Seisakusho
• Inventors: Sei, Masahiro
• Primary Examiner(s): Lacey, David L.
• Assistant Examiner(s): Edwards, Newton
• Application Number:
  US07/490,580
• Time in Patent Office: 488 Days
• Field of Search: 435/284, 435/289, 435/290, 435/311, 435/291, 435/240.4, 435/240.45, 47/60, 47/58, 47/61, 47/62, 47/63, 47/64, 47/65, 47/DIG. 6
• US Class Current: 435/292.1
• CPC Class Codes: A01H 4/001 Culture apparatus for tissue culture
• CPC Class Codes: Y10S 47/06 Plant growth regulation by control of light thereon

Abstract

The seedling breeding apparatus according to the present invention comprises a culture box including a nutrient medium support, a removable sterilizable germ-entrance preventive joint connected at one end to the culture box, a gas feeding system and a nutrient medium solution feeding system, both being connected to the culture box through the germ entrance-preventive joint. Therefore, the culture box and the germ entrance-preventive joint can be separated from the feeding systems and sterilized and hence sterilization is easy.

Claims

1. A seedling breeding apparatus comprising:

a culture unit including a culture box which includes a nutrient medium support capable of holding a nutrient-solution culture medium;

gas feeding means for feeding a gas to the culture box;

gas discharging means for discharging a gas from the culture box;

a nutrient solution feeding system including a nutrient solution reservoir tank for feeding a culture solution nutrient medium to the culture box;

a nutrient-solution discharging system for discharging a useless nutrient-solution nutrient medium out of the culture box; and

a germ entrance-preventive joint means connecting the culture box and the gas feeding means, gas discharging means and nutrient solution feeding and discharging systems and including means for sterilizing the joint means, wherein the feed gas and culture-solution nutrient medium are fed and the discharged gas and useless nutrient-solution nutrient medium are discharge in accordance with the degree of growth of cultured tissues bedded on the nutrient medium support.

2. A seedling breeding apparatus according to claim 1, wherein the germ entrance-preventive joint means comprises at least one introducing pipe which communicates at one end with the culture box and is open at its other end, said other end having a first flange and a first valve provided the pipe for blocking the inner passage of the pipe;

a discharging pipe having at one end a second flange coinciding with the first flange of the introducing pipe and a second valve provided in the discharging pipe for blocking the passage in the discharging pipe, the discharging pipe communicating at its other end with the gas feeding means and nutrient solution feeding and discharging systems;

hermetically sealing means for forming a surrounded hermetically sealed space in which the opening ends of the introducing and discharging pipes extend into and are spaced close to each other so as to form a slight gap therebetween;

means for sterilizing the inside of the hermetically sealing means;

tightening means for causing the first and second flanges to contact each other closely while maintaining the hermetically sealed space,

whereby the open ends of the respective pipes are sterilized by the sterilizing means, and the introducing and discharging pipes are then put into close contact with each other in communication relationship.

3. A seedling breeding apparatus according to claim 2, wherein the germ entrance-preventive joint means comprises:

a pipe section for introducing a gas;

a pipe section for discharging a gas;

a pipe section for introducing a nutrient solution; and

a pipe section for discharging a nutrient solution;

the nutrient solution introducing pipe being connected to a liquid reservior provided in the culture box;

means for atomizing the nutrient solution being fed to the culture box.

4. A seedling breeding apparatus according to claim 2, wherein the germ entrance-preventive joint means comprises:

a pipe section for introducing a gas;

a pipe section for discharging a gas;

a pipe section for introducing a nutrient solution;

a pipe section for discharging a nutrient solution;

the nutrient solution introducing pipe being connected to a liquid reservior provided in the culture box; and

means for airing the nutrient solution while being fed to the culture box.

5. A seedling breeding apparatus according to claim 3 wherein at least two of the four pipes sections are defined by a common pipe which includes a changeover valve positioned at an end of the common pipe.

6. A seedling breeding apparatus according to claim 2, including a capillary provided at the bottom of the culture box such that the capillary contacts the nutrient medium support and the pipe for discharging nutrient solution;

and

a nutrient solution discharge expediting means for controlling the pressure within the culture box such that it is slightly larger than the pressure in the discharging pipe.

7. A seedling breeding apparatus according to claim 1, wherein the nutrient solution feeding system includes a plurality of nutrient solution reservoir tanks;

a common feeding line connected through a valve to each of said nutrient solution reservoir tanks and connected to the germ entrance-preventive joint means; and

a wash line connected to the common feeding line.

8. A seedling breeding apparatus according to claim 1, wherein the nutrient solution feeding system comprises:

a solution feeding unit including a plurality of solution reservoir tanks for containing a like number of kinds of solutions;

means for feeding a PH adjusted liquid;

means for feeding a sterilized water for density adjustment;

mixing means for selecting and mixing desired solutions from the solution reservoir tanks and adjusting the PH and density of the mixed solutions to provide a desired solution; and

nutrient solution feeding means including a plurality of nutrient solution tanks for storing the like number of nutrient solutions obtained in the mixing means and feeding the solutions to the culture box.

9. A seedling breeding apparatus according to claim 1, wherein the culture unit includes a plurality of culture boxes.

10. A seedling breeding apparatus according to claim 1, wherein the culture box includes therein a flow generating fan means for imparting a wind stress to the cultured tissues therein to expedite acclimation.

11. A seedling breeding apparatus according to claim 4 wherein at least two of the four pipe sections are defined by a common pipe which is switched by a changeover valve positioned at an end of the common pipe.