Liquid transport system

• US 5,051,237 A
• Filed: 06/23/1988
• Issued: 09/24/1991
• Est. Priority Date: 06/23/1988
• Status: Expired due to Term

• Current Assignee: Behringwerke Aktiengesellschaft (Sanofi )
• Original Assignee: P B Diagnostic Systems Incorporated
• Inventors: Grenner, Gerd, Inbar, Shai, Long, Ernest W.
• Primary Examiner(s): WARDEN, JILL ALICE
• Assistant Examiner(s): Johnston, Jill
• Application Number:
  US07/210,732
• Time in Patent Office: 1,188 Days
• Field of Search: 422/56, 422/57, 422/58, 422/102, 436/169, 436/170
• US Class Current: 422/412
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements

Abstract

A liquid transport system provides controlled flow of liquid in a liquid flow zone between two surfaces wherein one surface includes a plurality of projections, or raised areas, arranged substantially throughout the surface in the liquid flow zone and an aperture for allowing the introduction of liquid between the two surfaces. In a preferred embodiment the projections on the first surface are arranged in parallel spaced rows and parallel spaced columns. The second surface may be one surface of a layer of a diagnostic assay element.

Claims

1. A diagnostic assay device comprising a first member and a diagnostic assay element, said first member and said assay element having opposed surfaces which are spaced apart throughout an intended liquid transport zone a distance effective to cause capillary flow of a sample liquid introduced therebetween throughout the intended liquid transport zone and means to permit introduction of a liquid between the opposed surfaces of said first member and said assay element, said opposed surface of said first member carrying a plurality of discrete noncontinuous projections arranged throughout said surface in the intended liquid transport zone, each said projection being spaced apart from the others in a predetermined pattern and being in contact or virtual contact with said opposed surface of said assay element.

2. The diagnostic assay device as defined in claim 1 wherein said projections are arranged in an ordered pattern of parallel spaced rows and parallel spaced columns.

3. The diagnostic assay element as defined in claim 1 wherein said projections are from about 50 to about 150 microns in height.

4. The diagnostic assay element as defined in claim 1 wherein said first member comprises a material which is impervious to the liquid to be assayed.

5. The diagnostic assay device as defined in claim 1 wherein said means to permit introduction of liquid comprises an aperture in said first member.

6. The diagnostic assay device as defined in claim 1 further including at least one liquid directing element extending from said aperture into contact or virtual contact with said opposed surface of said assay element.

7. The diagnostic assay device as defined in claim 5 wherein said diagnostic assay element comprises at least one reagent layer carried by a support.

8. The diagnostic assay device as defined in claims 7 wherein said support is transparent to wavelengths of radiation which are utilized to obtain a signal which is a function of an analyte in a liquid sample.

9. The diagnostic assay device as defined in claim 8 wherein said diagnostic assay element comprises a support carrying a reagent layer which in turn carries a light blocking layer.

10. The diagnostic assay device as defined in claim 9 wherein said reagent layer comprises an immunocomplex of a labeled analyte complexed to an immobilized binding partner.

11. The diagnostic assay device as defined in claim 10 wherein said label is a fluorescent moiety.

12. The diagnostic assay device as defined in claim 1 wherein said diagnostic asssay element comprises at least one reagent layer carried by a support.