Heterobifunctional maleimido containing coupling agents

• US 5,053,520 A
• Filed: 10/01/1989
• Issued: 10/01/1991
• Est. Priority Date: 09/22/1988
• Status: Expired due to Term

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Bieniarz, Christopher, Welch, Christopher J., Barnes, Grady
• Primary Examiner(s): LEE, LESTER
• Application Number:
  US07/402,012
• Time in Patent Office: 730 Days
• Field of Search: 548/520, 548/521, 530/331, 530/330, 530/329, 530/328, 530/390, 530/391
• US Class Current: 548/520
• CPC Class Codes: C07D 207/46 with hetero atoms directly attached to the ring nitrogen atom
• CPC Class Codes: C07K 17/06 attached to the carrier via a bridging agent
• CPC Class Codes: C12N 11/06 attached to the carrier via a bridging agent

Abstract

Novel polyamino acid based coupling agents are disclosed. These reagents are useful for conjugating proteins (e.g. antibodies to enzymes) for use in diagnostic assays.

Claims

1. A heterobifunctional reagent for linking two proteins comprising:

##STR17## wherein X is amino acid having from three to ten carbon atoms in a straight chain;

R is cycloaklyl or alkyl-cycloalkyl; and

n is from three to ten.

2. The reagent of claim 1 wherein R is cyclohexylmethyl.

3. The reagent of claim 1 wherein n is from two to five.

4. The reagent of claim 3 wherein n is three.

5. The reagent of claim 1 wherein X is a polyamide formed from repeated units of 6-aminocaproic acid.

6. The reagent of claim 1 comprising a compound of the formula:

##STR18##