Electrophoretic media

• US 5,055,517 A
• Filed: 03/31/1989
• Issued: 10/08/1991
• Est. Priority Date: 04/29/1988
• Status: Expired due to Term

• Current Assignee: Cambrex Bio Science Rockland, Inc.
• Original Assignee: At Biochem
• Inventors: Shorr, Robert, Jain, Tikam
• Primary Examiner(s): Schofer, Joseph L.
• Assistant Examiner(s): Sweet, Mark D.
• Application Number:
  US07/331,222
• Time in Patent Office: 921 Days
• Field of Search: 524/813, 524/833, 524/845, 524/916
• US Class Current: 524/813
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture
• CPC Class Codes: Y10S 524/916 Hydrogel compositions

Abstract

Electrophoretic media based on polymers with novel structures are disclosed. In one preferred embodiment, the polymers are formed by cross-linking polymerization of N,N-dimethylacrylamide with ethyleneglycol methacrylate. In another preferred embodiment, the polymers are formed by cross-linking polymerization of N,N-dimethylacrylamide and hydroxyethylmethacrylate with N,N-dimethylbisacrylamide.

Claims

1. An electrophoretic medium comprising an aqueous gel consisting essentially of the product formed by crosslinking polymerization in the presence of aqueous medium and in the absence of oxygen of

one or more monomers selected from compounds of the formula ##STR70## where R=alkyl, optionally mono-substituted with --OH or with --C(O)CH₂ C(O)CH₃ ;

R₁ =H or alkyl, optionally mono-substituted with--OH or with --C(O)CH₂ C(O)CH₃ ; and

one or more cross-linking agents selected from compounds of the formula ##STR71## where m=an integer of 1 or more;

R₂ =methyl; and

X is selected from O and S.

2. An electrophoretic medium of claim 1 where R₁ is other than H.

3. An electrophoretic medium of claim 1 where R=R₁.

4. An electrophoretic medium of claim 3 where R and R₁ are methyl.

5. An electrophoretic medium of claim 1 where R₂ =H.

6. An electrophoretic medium of claim 1 where the crosslinking agent is ethyleneglycol dimethacrylate.

7. An electrophoretic medium of claim 1 where the monomer is N,N-dimethylacrylamide and the cross-linking agent is ethyleneglycol dimethacrylate.

8. An electrophoretic medium of claim 7 which further comprises a polyethylenehydroxyamine.

9. An electrophoretic medium of claim 7 which further comprises about 0.01 to 2% of a polyoxyethylene having a molecular weight of about 2000.

10. An electrophoretic medium of claim 7 which further comprises a buffer selected from the group consisting of tris/borate/EDTA, tris/acetate/EDTA, tris/phosphate/EDTA and tris/glycylglycine.

11. An electrophoretic medium of claim 7 which further comprises a polyethylenehydroxyamine, about 0.01 to 2% of a polyoxyethylene having a molecular weight of about 2000, and a buffer selected from the group consisting of tris/borate/EDTA, tris/acetate/EDTA, tris/phosphate/EDTA and tris/glycylglycine.

12. An electrophoretic medium of claim 1 which further comprises one or more additives selected from urea, glycerol and polymethylenehydroxyamines.

13. An electrophoretic medium of claim 1 which further comprises a denaturing agent.

14. An electrophoretic medium of claim 13 where said denaturing agent is selected from sodium dodecyl sulfate and polyoxyethylenes.

15. An electrophoretic medium comprising beads formed by crosslinking polymerization of

one or more monomers selected from compounds of the formula ##STR72## where R=alkyl, optionally mono-substituted with --OH or with --C(O)CH₂ C(O)CH₃ ;

R₁ =H or alkyl, optionally mono-substituted with--OH or with --C(O)CH₂ C(O)CH₃ ; and

one or more cross-linking agents selected from compounds of the formula ##STR73## where m=an integer of 1 or more;

R₂ =methyl; and

X is selected from O and S.