Fluorescent conjugates for analysis of molecules and cells

• US 5,055,556 A
• Filed: 10/25/1985
• Issued: 10/08/1991
• Est. Priority Date: 10/06/1981
• Status: Expired due to Fees

• Current Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Original Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Inventors: Glazer, Alexander N., Stryer, Lubert, Oi, Vernon T.
• Primary Examiner(s): Brown, Johnnie R.
• Assistant Examiner(s): Jay, Jeremy M.
• Application Number:
  US06/791,248
• Time in Patent Office: 2,174 Days
• Field of Search: 436/501, 436/547, 436/800, 436/805, 530/370, 530/387, 530/388, 530/389, 530/807
• US Class Current: 530/370
• CPC Class Codes: G01N 33/533 with fluorescent label
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

Sensitive detection techniques and compositions for such techniques are provided by employing fluorescent proteins having bilin prosthetic groups as labels. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjunction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.

Claims

1. A receptor covalently bound to a phycobiliprotein.

2. A receptor according to claim 1, wherein said receptor is a cell surface protein receptor.

3. A receptor according to claim 1, wherein said receptor is avidin.

4. A receptor according to claim 1, wherein said receptor is an immunoglobulin.