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Purification of nerve growth factor (.beta. subunit) by subunit exchange chromatography

  • US 5,057,223 A
  • Filed: 06/28/1990
  • Issued: 10/15/1991
  • Est. Priority Date: 07/20/1989
  • Status: Expired due to Term
First Claim
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1. A method for the purification of the β

  • subunit of a Nerve Growth Factor which comprises;

    a) covalent binding a first β

    subunit of Nerve Growth Factor (NGF β

    subunit) to a solid matrix having a coupling density sufficient to achieve subunit exchange chromatography;

    b) combining the matrix having said first NGF (β

    subunit) immobilized thereon with a preparation containing a second β

    subunit of Nerve Growth Factor (NGF β

    subunit), whereby said second NGF (β

    subunit) is bound to said matrix;

    c) eluting said matrix with a releasing agent to release said second NGF (β

    subunit) from said matrix; and

    d) recovering said released second NGF (β

    subunit).

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