Saponin adjuvant

• US 5,057,540 A
• Filed: 08/27/1990
• Issued: 10/15/1991
• Est. Priority Date: 05/29/1987
• Status: Expired due to Term

• Current Assignee: Antigenics Inc. (Agenus Inc.)
• Original Assignee: Cambridge Biotech Corp. (Agenus Inc.)
• Inventors: Kensil, Charlotte A., Marciani, Dante J.
• Primary Examiner(s): Griffin, Ronald W.
• Assistant Examiner(s): Carson, Nancy S.
• Application Number:
  US07/573,268
• Time in Patent Office: 414 Days
• Field of Search: 514/25, 514/26, 514/33, 514/35, 514/885, 424/88, 424/89, 424/195.1, 536/4.1, 536/6.3, 536/18.1, 536/127, 536/128, 536/5
• US Class Current: 424/278.1
• CPC Class Codes: A61K 2039/55577 Saponins; Quil A; QS21; ISCOMS
• CPC Class Codes: A61K 39/39 characterised by the immunostimulating additives, e.g. chemical adjuvants
• CPC Class Codes: C07H 15/256 Polyterpene radicals
• CPC Class Codes: C07J 17/005 Glycosides

Abstract

Substantially pure saponins are disclosed. The saponins of the present invention are useful as immune adjuvants. Disclosed as well are immune response-provoking compositions comprising an antigen in admixture with the substantially pure saponins.

Claims

1. Substantially pure saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by a single predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, when analyzed on reverse phase-HPLC on a Vydac C₄ column having 5 μ

m particle size, 330 Å

pore, 4.6 mm ID×

25 cm L in a solvent of 40 mM acetic acid in methanol/water (58/42;

v/v) at a flow rate of 1 ml/mixture, and wherein said saponin has immune adjuvant activity and is less toxic when used as an adjuvant than said Quillaja saponaria extract.

14. A method of enhancing an immune response to an antigen in an individual comprising administration of an amount of the substantially pure saponin adjuvants from any of claims 1-7 and 8-13 to said individual in an amount sufficient to enhance the immune response of said individual to said antigen.

15. A pharmaceutical composition useful for inducing the production of antibodies to an antigen in an individual comprising an immunogenically effective amount of an antigen and at least one substantially pure saponin as in any one of claims 1-7 and 8-13, wherein said substantially pure saponin is present in an amount sufficient to enhance the immune response of said individual to said antigen.

16. The pharmaceutical composition of claim 15, wherein said individual is a mammal.

2. Substantially pure QA-7 saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by one predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, and having a retention time of approximately 9-10 minutes when analyzed on reverse phase HPLC on a Vydac C₄ column having 5 μ

m particle size, 330 Å

pore, 4.6 mm ID×

25 cm L in a solvent of 40 mM acetic acid in methanol/water (58/42;

v/v) at a flow rate of 1 ml/minute.

3. The substantially pure QA-7 saponin of claim 2, wherein said saponin has immune adjuvant activity, and wherein said saponin is characterized by a carbohydrate content of about 35% per dry weight as assayed by anthrone, has a UV adsorption maxima of 205-210 nm, has a micellar concentration of 0.06% (w/v) in water and 0.07% in phosphate buffered saline, and causes no detectable hemolysis of sheep red blood cells at concentrations of 200 μ

g/ml.

4. The substantially pure QA-7 saponin of claim 3, wherein said carbohydrate content has a composition comprising the monosuccharides:

terminal rhamnose, terminal xylose, terminal glucose, terminal galactose, 3-xylose, 3,4-rhamnose, 2,3-fucose, 2,3-glucuronic acid and apiose.

5. Substantially pure QA-21 saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by one predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, and having a retention time of approximately 51minutes when analyzed on reverse phase-HPLC on a Vydac C₄ column having 5 μ

m particle size, 330 Å

pore, 4.6 mm ID×

25 cm L in a solvent of 40 mM acetic acid in methanol/water (58/42;

v/v) at a flow rate of 1 ml/minute.

6. The substantially pure QA-21 saponin of claim 5, wherein said saponin has immune adjuvant activity, and wherein said saponin is characterized by a carbohydrate content of about 22% per dry weight as assayed by anthrone, has a UV absorption maxima of 205-210 nm, has a micellar concentration of about 0.03% (w/v) in water and 0.02% (w/v) in phosphate buffered saline, and causes hemolysis of sheep red blood cells at concentrations of 25 μ

g/ml or greater.

7. The substantially pure QA-21 saponin of claim 6, wherein said carbohydrate content has a composition comprising the monosaccharides:

terminal rhamnose, terminal arabinose, terminal apiose, terminal xylose, 4-rhamnose, terminal glucose, terminal galactose, 2-fucose, 3-xylose, 3,4-rhamnose and 2,3-glucuronic acid.

8. A substantially pure QA-17 saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by one predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, and having a retention time of approximately 35 minutes on reverse phase-HPLC on a Vydac C₄ column having 5 μ

m particle size, 330 Å

pore, 4.6 mm ID×

25 cm L in a solvent of 40 mN acetic acid in methanol/water (58/42;

v/v) at a flow rate of 1 ml/minute.

9. The substantially QA-17 saponin of claim 8, wherein said saponin has immune adjuvant activity, and wherein said saponin is characterized by a carbohydrate content of about 29% per dry weight as assayed by anthrone, has a UV absorption maxima of 205-210 nm, has a micellar concentration of about 0.06% (w/v) in water and 0.03% (w/v) in phosphate-bufferred saline, and causes hemolysis of sheep red blood cells at concentrations of 25 μ

g/ml.

10. The substantially pure QA-17 saponin of claim 9, wherein said carbohydrate content has a composition comprising the monosaccharides:

terminal rhamnose, terminal xylose, 2-fucose, 3-xylose, 3,4-rhamnose, 2,3-glucuronic acid, terminal glucose, 2-arabinose, terminal galactose and apiose.

11. A substantially pure QA-18 saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by one predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, and having a retention time of approximately 38 minutes on reverse phase-HPLC on a Vydac C₄ column having 5 μ

m particle size, 330 Å

pore, 4.6 mm ID×

25 cm L in a solvent of 40 mM acetic acid in methanol/water (58/42;

v/v) at a flow rate of 1 ml/minute.

12. The substantially QA-18 saponin of claim 11, wherein said saponin has immune adjuvant activity, and wherein said saponin is characterized by a carbohydrate content of about 25-26% per dry weight as assayed by anthrone, has a UV absorption maxima of 205-210 nm, has a micellar concentration of 0.04% (w/v) in water and 0.02% (w/v) in phosphate-buffered saline, and causes hemolysis of sheep red blood cells at concentrations of 25 μ

g/ml.

13. The substantially pure QA-18 saponin of claim 12, wherein said carbohydrate content has a composition comprising the monosaccharides:

terminal rhamnose, terminal arabinose, terminal apiose, terminal xylose, terminal glucose, terminal galatose, 2-fucose, 3-xylose, 3,4-rhamnose and 2,3-glucuronic acid.