Method for separating nucleic acids and nucleic acid probes
First Claim
1. A method of separating nucleic acid from substances to which it is bound non-specifically comprising:
- a) contacting said nucleic acid and said substances to which it is bound non-specifically with support medium; and
applying alternate electric field and direct electric field to said support medium;
wherein the direction of said direct field is essentially parallel to the direction of said alternating field.
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Accused Products
Abstract
An improved method and device for nucleic acid hybridization assay employing combined direct an alternating field electrophoresis are disclosed. In the method of the present invention, a sample is hybridized with nucleic acid probe and is contacted with a support medium where direct and alternating electric fields are applied. Under the influence of the electric fields, hybrid separates from non-specifically bound nucleic acid probe. The hybrid may be measured on the support medium itself as on a paper strip or in a cartridge containing support medium or may be blotted on an inert surface and then measured. The method an device are useful in the diagnosis of diseases. Kits are provided for assay of a large number of diseases.
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Citations
2 Claims
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1. A method of separating nucleic acid from substances to which it is bound non-specifically comprising:
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a) contacting said nucleic acid and said substances to which it is bound non-specifically with support medium; and applying alternate electric field and direct electric field to said support medium;
wherein the direction of said direct field is essentially parallel to the direction of said alternating field.
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2. A method of separating nucleic acid from substances to which it is bound non-specifically comprising:
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a) contacting said nucleic acid and said substances to which it is bound non-specifically with support medium; and b) applying alternate electric field and direct electric field to said support medium;
wherein said direct electric field and said alternating electric field are applied at different times to said support medium.
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Specification