Affinity matrices of modified polysaccharide supports
First Claim
1. A modified polysaccharide material which comprises:
- (1) a water-insoluble polysaccharide covalently bonded to a synthetic polymer;
(2) said synthetic polymer made from(a) a polymerizable compound which has an epoxy group capable of direct covalent coupling to said polysaccharide; and
(b) one or more polymerizable compounds containing(i) a chemical group capable of causing the covalent coupling of said synthetic polymer to an affinity ligand or a biologically active molecule, or(ii) a hydrophobic chemical group.
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Abstract
The invention is directed to a modified polysaccharide material which comprises: (1) polysaccharide covalently bonded to a synthetic polymer; (2) the synthetic polymer being made from (a) a polymerizable compound which is capable of being covalently coupled directly or indirectly to said polysaccharide, and (b) one or more polymerizable compounds containing (i) a chemical group capable of causing the covalent coupling of the compound (b) to an affinity ligand or a biologically active molecule or (ii) a hydrophobic compound.
The invention is also directed to devices for the chromatographic separation of at least two components of a mixture comprising the modified polysaccharide material of the invention, wherein the device is configured for radial or tangential flow.
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Citations
28 Claims
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1. A modified polysaccharide material which comprises:
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(1) a water-insoluble polysaccharide covalently bonded to a synthetic polymer; (2) said synthetic polymer made from (a) a polymerizable compound which has an epoxy group capable of direct covalent coupling to said polysaccharide; and (b) one or more polymerizable compounds containing (i) a chemical group capable of causing the covalent coupling of said synthetic polymer to an affinity ligand or a biologically active molecule, or (ii) a hydrophobic chemical group. - View Dependent Claims (2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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7. The material of claim I wherein said chemical group capable of causing the covalent coupling of said synthetic polymer to said affinity ligand is selected from the group consisting of a primary amine and an aldehyde.
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19. A process for effecting chromatographic separation of at least two components of a sample comprising contacting said sample with a self-supporting fibrous matrix comprising a modified polysaccharide material, said modified polysaccharide material comprising:
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(1) a water insoluble polysaccharide covalently bonded to a synthetic polymer; (2) said synthetic polymer comprising (a) a polymerizable compound which has an epoxy group capable of direct covalent coupling to said polysaccharide; and (b) one or more polymerizable compounds containing (i) a chemical group capable of causing the covalent coupling of said synthetic polymer to an affinity ligand or a biologically active molecule, or (ii) a hydrophobic chemical group. - View Dependent Claims (20, 21)
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22. A chromatographic device for effecting chromatographic separation of at least two components of a sample, comprising:
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(A) a modified polysaccharide material which comprises; (1) a water-insoluble polysaccharide covalently bonded to a synthetic polymer; (2) said synthetic polymer made from (a) a polymerizable compound which has an epoxy group capable of direct covalent coupling to said polysaccharide; and (b) one or more polymerizable compounds containing (i) a chemical group capable of causing the covalent coupling of said synthetic polymer to an affinity ligand or a biologically active molecule, or (ii) a hydrophobic chemical group; and (B) means for effecting tangential flow of said sample across said modified polysaccharide material. - View Dependent Claims (23)
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24. A chromatography device for effecting chromatographic separation of at least two components of a sample, comprising:
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(1) a cylindrical core, (2) at least a first and second stationary phases wound around said cylindrical core, wherein (a) a first phase comprises a modified polysaccharide material which comprises; (i) a water-insoluble polysaccharide covalently bonded to a synthetic polymer; (ii) said synthetic polymer made from (1) a polymerizable compound which has an epoxy group capable of direct covalent coupling to said polysaccharide; and (2) one or more polymerizable compounds containing (a) a chemical group capable of causing the covalent coupling of said synthetic polymer to an affinity ligand or a biologically active molecule, or (b) a hydrophobic chemical group, wherein said first phase is in the form of a sheet; (b) a second phase comprising a means for supporting, separating, and providing channels tangential to said cylindrical core; (3) means for distributing the sample to said channels; (4) means for collecting the sample from said channels; and (5) a cylindrical housing. - View Dependent Claims (25, 26, 27, 28)
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Specification