Sterile transfer system
First Claim
1. A method for delivering one or more aliquots of a sterile solution into a sterile receptacle without contamination of said sterile solution, said method comprising the steps of:
- a) providing a packaged solution held within a gas-pressurized or pressure-activated container, said container having a valve with a first inner channel, an actuator, wherein said actuator cooperates with said valve to allow opening and closing of said valve, and a separate elongated delivery conduit having a second inner channel, wherein said valve and actuator are positioned between said container and said delivery conduit, the actuator cooperating with the valve and conduit to connect said first and second inner channels,b) sterilizing said solution, the inside part of the container and a portion of the outside of the container including said valve, said actuator, and said delivery conduit,c) positioning said delivery conduit in relationship to said receptacle to cause liquid from said second inner channel to enter said receptacle when said valve is opened, andd) opening said valve with said actuator, whereby said sterile solution is forced through said first and second inner channels and then enters said sterile receptacle.
1 Assignment
0 Petitions
Accused Products
Abstract
Apparatus and method for delivering one or more aliquots of a sterile solution into a sterile receptacle without contamination of the sterile solution, the method including the steps of: a) providing a packaged solution held within a gas-pressurized or pressure-activated container, the container having a valve with a first inner conduit, an actuator which cooperates with the valve to allow opening and closing of the valve, and a delivery conduit having a second inner channel, wherein the valve and actuator are positioned between the container and the delivery conduit, the actuator cooperating with the valve and conduit to connect the first and second inner channels, b) sterilizing the solution, and at least the inside part of the container and a portion of the outside of the container including the valve, the actuator, and the delivery conduit, c) positioning the delivery conduit in relationship to the receptacle to cause liquid from the second inner channel to enter the receptacle when the valve is opened, and d) opening the valve with the actuator, whereby the sterile solution is forced through the first and second inner channels and then enters the sterile receptacle.
160 Citations
19 Claims
-
1. A method for delivering one or more aliquots of a sterile solution into a sterile receptacle without contamination of said sterile solution, said method comprising the steps of:
-
a) providing a packaged solution held within a gas-pressurized or pressure-activated container, said container having a valve with a first inner channel, an actuator, wherein said actuator cooperates with said valve to allow opening and closing of said valve, and a separate elongated delivery conduit having a second inner channel, wherein said valve and actuator are positioned between said container and said delivery conduit, the actuator cooperating with the valve and conduit to connect said first and second inner channels, b) sterilizing said solution, the inside part of the container and a portion of the outside of the container including said valve, said actuator, and said delivery conduit, c) positioning said delivery conduit in relationship to said receptacle to cause liquid from said second inner channel to enter said receptacle when said valve is opened, and d) opening said valve with said actuator, whereby said sterile solution is forced through said first and second inner channels and then enters said sterile receptacle. - View Dependent Claims (2, 4, 5, 8, 9)
-
-
3. A method for manufacture of a gas pressurized or pressure activated device, comprising the steps of:
-
providing a gas-pressurized or pressure-activated container comprising a packaged solution, a cap, a valve, an actuator, wherein said actuator cooperates with said valve to allow opening and closing of said valve, b) sterilizing said solution, valve, actuator and delivery conduit, c) covering said sterilized valve and actuator with said cap wherein said cap is positioned to prevent contamination of said valve and said actuator, and d) covering said delivery conduit to prevent contamination of said delivery conduit. - View Dependent Claims (6, 7)
-
-
10. A liquid delivery kit comprising:
-
a gas-pressurized or pressure-activated container comprising a sterile liquid, a sterile valve, and actuator and a cap positioned to maintain the sterility of said valve and actuator, wherein said actuator cooperates with said valve to allow opening and closing of said valve, and a separate elongated sterile delivery conduit sized and shaped to cooperate with said actuator to connect inner portions of said valve and conduit to allow sterile delivery of said liquid from said container to a desired location, wherein said sterile conduit, valve, and actuator together comprise fewer microorganisms than are necessary to cause contamination of a cell culture medium. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
-
-
19. A pressurized graduated container comprising a sterile liquid said liquid being chosen from a tissue culture solution, a microbiological cell culture solution and other cell culture related solutions for use with living cells.
Specification