Apparatus for conducting measurements of electrochemiluminescent phenomena

• US 5,061,445 A
• Filed: 11/03/1988
• Issued: 10/29/1991
• Est. Priority Date: 11/03/1988
• Status: Expired due to Term

• Current Assignee: BioVeris Corporation (Roche Holding AG)
• Original Assignee: IGEN, Inc. (Teligent Incorporated)
• Inventors: Woodward, Steve, Zoski, Glenn
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Santiago, Amalia L.
• Application Number:
  US07/267,234
• Time in Patent Office: 1,090 Days
• Field of Search: 350/332, 350/331 R, 422/52, 328/181, 328/185, 307/228, 340/781, 250/361 C
• US Class Current: 422/52
• CPC Class Codes: G01N 21/66 electrically excited, e.g. electroluminescence
• CPC Class Codes: H03K 4/026 using digital techniques

Abstract

An apparatus for conducting measurements of electrochemiluminescent (ECL) phenomena includes a cell unit having an electrode configuration for inducing the emission of ECL light by the application of a selected voltage waveform to a sample fluid including an ECL moiety. The sample fluid is transported to and from the cell unit by a flow-through pump/tubing system. A photomultiplier tube detects the intensity of light emitted by the sample fluid during the ECL measurement process. A computer control unit both analyzes the detected data and provides digital control signals to the cell unit to generate effective voltage waveforms. The digital control signals are supplied to a novel pulse width modulated digital to analog converter which outputs a ramp voltage waveform having the desired slope.

Claims

1. Apparatus for conducting measurements of electrochemiluminescent phenomena, comprising:

cell means for holding a sample fluid which contains an electrochemiluminescent moiety;

detector means for detecting electromagnetic radiation emitted by said electrochemiluminescent moiety in said sample fluid and providing a signal indicative of the intensity of emitted radiation;

control means for generating a digital control signal indicative of a selected slope of a ramp voltage waveform;

digital to analog converter means for generating said ramp voltage waveform in response to said digital control signal;

said digital to analog converter means comprising pulse width modulation means for converting said digital control signal to a pulse width modulated control signal; and

integrator means for producing said ramp voltage waveform whose slope is determined by said pulse width modulated control signal produced by said pulse width modulation means; and

means for supplying electrochemical energy to said sample fluid in response to the ramp voltage waveform such that said electrochemiluminescent moiety in said sample fluid is induced to repeatedly emit electromagnetic radiation.

2. Apparatus according to claim 1 wherein said control means comprises means for generating a second digital control signal indicative of a second voltage waveform composed of at least one constant voltage, said apparatus further comprising second digital-to-analog converter means for generating said second voltage waveform in response to said second digital control signal, and wherein the means for supplying electrochemical energy to said sample fluid in response to the ramp voltage waveform comprises means for supplying electrochemical energy to said sample fluid in response to the second voltage waveform.

3. Apparatus according to claim 1 further comprising fluid transport means for transporting said sample fluid to and from said cell means.

4. Apparatus according to claim 1 wherein said control means includes programmable digital computing means for generating said digital control signal.

5. Apparatus according to claim 1 wherein said pulse width modulation means includes programmable timer means for providing a first pulse width modulated pulse train in response to the digital control signal indicative of said selected slope of said ramp voltage waveform;

first and second voltage sources defining upper and lower pulse amplitudes, respectively; and

multiplexer means connected to said first and second voltage sources and responsive to said first pulse width modulated pulse train for producing said pulse width modulated control signal as a second pulse width modulated pulse train having at least one of said upper and lower pulse amplitudes.

6. Apparatus according to claim 5 wherein said control means is operative to supply said digital control signal having load values and hold values and wherein said programmable timer means includes sixteen bit counter means including a sixteen bit load register and a sixteen bit hold register for holding said load and hold values, respectively, the duty cycle of each pulse within said first pulse width modulated pulse train being equal to said load value divided by the sum of said load and hold values, whereby the resolution of said digital to analog converter means is greater than sixteen bits.

7. Apparatus according to claim 1 wherein said detector means includes light detector means for detecting photons of said electromagnetic radiation emitted by said electrochemiluminescent moiety in said sample fluid and providing an output pulse in response to each detected photon, and counter means responsive to said output pulses for providing said signal indicative of the intensity of emitted radiation.

8. Apparatus according to claim 7 wherein said signal indicative of the intensity of emitted radiation is a digital signal.

9. Apparatus according to claim 7 wherein said light detector means includes photomultiplier tube means.

10. Apparatus according to claim 7 wherein said counter means provides a first digital signal indicative of a count of a first fractional number of said output pulses and a second digital signal indicative of a count of a second fractional number of said output pulses.

11. Apparatus according to claim 10 further comprising output digital to analog converter means for converting a selected one of said first and second output digital signals to analog form.

12. Apparatus for conducting measurements of electrochemiluminescent phenoma, comprising:

cell means for holding a sample fluid which contains an electrochemiluminescent moiety;

means for introducing a sample fluid containing an electrochemiluminescent moiety to said cell means;

detector means for detecting electromagnetic radiation emitted by said electrochemiluminescent moiety in said sample fluid and providing a signal indicative of the intensity of emitted radiation;

ramp voltage generating means for generating a ramp voltage waveform having a selected slope, said ramp voltage generating means including means for producing a control signal representing said selected slope, and means for producing said ramp voltage waveform having said selected slope in response to said control signal; and

means for supplying electrochemical energy to said sample fluid in response to the ramp voltage waveform said that said electrochemiluminescent moiety in said sample fluid is induced to repeatedly emit electromagnetic radiation.

13. An apparatus as recited in claim 12 wherein said means for introducing a sample fluid to said cell means includes fluid transport means for introducing a sample to and from said cell means.

19. An apparatus as recited in claim 12, wherein said means for producing a control signal comprises means for producing a digital control signal representing said selected slope, and wherein said means for producing said ramp voltage waveform comprises means for producing said ramp voltage waveform having said selected slope in response to said digital control signal.

20. An apparatus as recited in claim 19, wherein said means for producing said ramp voltage waveform comprises means for producing a pulse train which is pulse width modulated in response to the digital control signal, and means for integrating said pulse train to produce said ramp voltage waveform.

14. Apparatus for conducting measurements of electrochemiluminescent phenomena, comprising:

cell means for holding a sample fluid which contains an electrochemiluminescent moiety;

detector means for detecting electromagnetic radiation emitted by said electrochemiluminescent moiety in said sample fluid and providing a signal indicative of the intensity of emitted radiation;

computer control means for generating a first predetermined voltage waveform having at least one selected slope;

said computer control means including means for producing a control signal representing said selected slope, and means for producing said first predetermined voltage waveform having said selected slope in response to said control signal; and

means for supplying electrochemical energy to said sample fluid in response to the first predetermined voltage waveform such that said electrochemiluminescent moiety in said sample fluid is induced to repeatedly emit electromagnetic radiation.

15. An apparatus as recited in claim 14 wherein said computer control means comprises means for generating a ramp voltage waveform as said first predetermined voltage waveform.

16. An apparatus as recited in claim 14 wherein said computer control means comprises means for generating a curved waveform as said first predetermined voltage waveform.

17. An apparatus as recited in claim 16 wherein said means for generating a curved waveform comprises means for generating a sinusoidal waveform.

18. An apparatus as recited in claim 15 wherein said computer control means comprises means for generating a second predetermined voltage waveform, and means for combining said ramp voltage waveform and said second predetermined voltage waveform.

21. An apparatus as recited in claim 14, wherein said means for producing a control signal representing said selected slope comprises computer means for producing a digital control signal representing said at least one selected slope;

and wherein said means for producing said first predetermined voltage waveform comprises means for producing said predetermined voltage waveform having said selected slope in response to said digital control signal.

22. An apparatus as recited in claim 21, wherein said means for producing said first predetermined voltage waveform comprises integrator means for producing said first predetermined voltage waveform in response to a control input signal supplied thereto representing said selected slope of said first predetermined voltage waveform;

and means for supplying said control input signal to said integrator means in response to said digital control signal such that said integrator means produces said first predetermined voltage waveform having said selected slope.